Distribution of Adenoviral Vector in Brain after Intravenous Administration

Chanil Moon, Cheil Moon, Wha Sun Kang, Dae Chul Jeong, Jong Youl Jin

Research output: Contribution to journalArticlepeer-review

Abstract

The delivery of transgenes to the central nervous system (CNS) can be a valuable tool to treat CNS diseases. Various systems for the delivery to the CNS have been developed; vascular delivery of viral vectors being most recent. Here, we investigated gene transfer to the CNS by intravenous injection of recombinant adenoviral vectors, containing green fluorescence protein (GFP) as a reporter gene. Expression of GFP was first observed 6 days after the gene transfer, peaked at 14 days, and almost diminished after 28 days. The observed expression of GFP in the CNS was highly localized to hippocampal CA regions of cerebral neocortex, inferior colliculus of midbrain, and granular cell and Purkinje cell layers of cerebellum. It is concluded that intravenous delivery of adenoviral vectors can be used for gene delivery to the CNS, and hence the technique could be beneficial to gene therapy.

Original languageEnglish (US)
Pages (from-to)108-111
Number of pages4
JournalJournal of Korean Medical Science
Volume18
Issue number1
StatePublished - Feb 2003

Keywords

  • Adenoviridae
  • Central Nervous System
  • Gene Therapy
  • Green Fluorescent Protein

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Distribution of Adenoviral Vector in Brain after Intravenous Administration'. Together they form a unique fingerprint.

Cite this