Distribution of β3-adrenoceptor mRNA in human tissues

Dan E. Berkowitz, Nancy A. Nardone, Richard M. Smiley, David T. Price, David K. Kreutter, Robert T. Fremeau, Debra A. Schwinn

Research output: Contribution to journalArticle

Abstract

The β3-adrenoceptor is a G protein-coupled receptor which mediates metabolic functions of the endogenous catecholamines epinephrine and norepinephrine. Questions exist regarding distribution of the β3-adrenoceptor in human tissue. In order to examine the distribution of β3-adrenoceptor mRNA in human tissues, we used sensitive and specific RNase protection assays without previous PCR amplification in an extensive list of human tissues. We confirm the presence of β3-adrenoceptor mRNA in human white fat from several locations, gall bladder, and small intestine, as well as extend the distribution of β3-adrenoceptor mRNA to previously uncharacterized human tissues such as stomach and prostate. The presence of β3-adrenoceptor mRNA in human white adipose tissue has important implications regarding possible use of β3-adrenoceptor selective agonists as anti-obesity agents, and the demonstration of β3-adrenoceptor mRNA in a number of gastrointestinal tissues and prostate raises the question of the role of the β3-adrenoceptor in motility and secretory processes.

Original languageEnglish (US)
Pages (from-to)223-228
Number of pages6
JournalEuropean Journal of Pharmacology: Molecular Pharmacology
Volume289
Issue number2
DOIs
StatePublished - Apr 28 1995

Keywords

  • (Human)
  • Adipose tissue
  • Gallbladdder
  • Obesity
  • RNase protection
  • β-Adrenoceptor

ASJC Scopus subject areas

  • Pharmacology

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    Berkowitz, D. E., Nardone, N. A., Smiley, R. M., Price, D. T., Kreutter, D. K., Fremeau, R. T., & Schwinn, D. A. (1995). Distribution of β3-adrenoceptor mRNA in human tissues. European Journal of Pharmacology: Molecular Pharmacology, 289(2), 223-228. https://doi.org/10.1016/0922-4106(95)90098-5