Distribution and synaptic localization of nicotinic acetylcholine receptors containing a novel α7 subunit isoform in embryonic rat cortical neurons

Emily G Severance, Javier Cuevas

Research output: Contribution to journalArticle

Abstract

Neuronal nicotinic acetylcholine receptors (nAChRs) containing the α7 subunit isoform, α7-2 (α7-2-nAChRs), have previously been found to form functional homopentameric channels that desensitize slowly and bind α-bungarotoxin (αBgt) in a rapidly reversible manner. This isoform incorporates a novel cassette exon in the extracellular, ligand binding domain of the native receptor. Although this α7 subunit isoform has been detected in peripheral ganglia as well as in the central nervous system, little is known about the cellular function of α7-2-nAChRs. Co-localization immunocytochemical studies were conducted in an embryonic rat cultured cortical neuron model using a polyclonal antibody (Ab 87) raised against the amino acid sequence of the cassette exon, in combination with (1) an antibody that recognizes all known α7-nAChRs, (2) αBgt, and (3) antibodies directed against multiple cellular markers. The pattern of α7-2-nAChR expression was consistent with α7 staining in general, based on co-distribution of mAb319 and αBgt signals. However, α7-2-nAChRs clearly represent a distinct subset of α7 receptors. The α7-2-nAChR subtype was found throughout the cell-soma surface and was localized to a subpopulation of dendrites. Punctate staining characteristic of synaptic α7-2 targeting was observed at post-synaptic densities and intermittently at pre-synaptic locations. The α7-2 subunit was expressed on both GABAergic and non-GABAergic neurons. These studies reveal that receptors containing the α7-2 subunit constitute a subpopulation of α7-nAChRs and likely participate in cell-to-cell signaling in developing synapses of central neurons.

Original languageEnglish (US)
Pages (from-to)104-109
Number of pages6
JournalNeuroscience Letters
Volume372
Issue number1-2
DOIs
StatePublished - Nov 30 2004
Externally publishedYes

Fingerprint

Nicotinic Receptors
Protein Isoforms
Neurons
Bungarotoxins
Antibodies
Exons
Staining and Labeling
Post-Synaptic Density
Carisoprodol
Dendrites
Ganglia
Synapses
Amino Acid Sequence
Central Nervous System
Ligands

Keywords

  • α-Bungarotoxin
  • GABA
  • Immunocytochemistry
  • Synapse

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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title = "Distribution and synaptic localization of nicotinic acetylcholine receptors containing a novel α7 subunit isoform in embryonic rat cortical neurons",
abstract = "Neuronal nicotinic acetylcholine receptors (nAChRs) containing the α7 subunit isoform, α7-2 (α7-2-nAChRs), have previously been found to form functional homopentameric channels that desensitize slowly and bind α-bungarotoxin (αBgt) in a rapidly reversible manner. This isoform incorporates a novel cassette exon in the extracellular, ligand binding domain of the native receptor. Although this α7 subunit isoform has been detected in peripheral ganglia as well as in the central nervous system, little is known about the cellular function of α7-2-nAChRs. Co-localization immunocytochemical studies were conducted in an embryonic rat cultured cortical neuron model using a polyclonal antibody (Ab 87) raised against the amino acid sequence of the cassette exon, in combination with (1) an antibody that recognizes all known α7-nAChRs, (2) αBgt, and (3) antibodies directed against multiple cellular markers. The pattern of α7-2-nAChR expression was consistent with α7 staining in general, based on co-distribution of mAb319 and αBgt signals. However, α7-2-nAChRs clearly represent a distinct subset of α7 receptors. The α7-2-nAChR subtype was found throughout the cell-soma surface and was localized to a subpopulation of dendrites. Punctate staining characteristic of synaptic α7-2 targeting was observed at post-synaptic densities and intermittently at pre-synaptic locations. The α7-2 subunit was expressed on both GABAergic and non-GABAergic neurons. These studies reveal that receptors containing the α7-2 subunit constitute a subpopulation of α7-nAChRs and likely participate in cell-to-cell signaling in developing synapses of central neurons.",
keywords = "α-Bungarotoxin, GABA, Immunocytochemistry, Synapse",
author = "Severance, {Emily G} and Javier Cuevas",
year = "2004",
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doi = "10.1016/j.neulet.2004.09.020",
language = "English (US)",
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TY - JOUR

T1 - Distribution and synaptic localization of nicotinic acetylcholine receptors containing a novel α7 subunit isoform in embryonic rat cortical neurons

AU - Severance, Emily G

AU - Cuevas, Javier

PY - 2004/11/30

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N2 - Neuronal nicotinic acetylcholine receptors (nAChRs) containing the α7 subunit isoform, α7-2 (α7-2-nAChRs), have previously been found to form functional homopentameric channels that desensitize slowly and bind α-bungarotoxin (αBgt) in a rapidly reversible manner. This isoform incorporates a novel cassette exon in the extracellular, ligand binding domain of the native receptor. Although this α7 subunit isoform has been detected in peripheral ganglia as well as in the central nervous system, little is known about the cellular function of α7-2-nAChRs. Co-localization immunocytochemical studies were conducted in an embryonic rat cultured cortical neuron model using a polyclonal antibody (Ab 87) raised against the amino acid sequence of the cassette exon, in combination with (1) an antibody that recognizes all known α7-nAChRs, (2) αBgt, and (3) antibodies directed against multiple cellular markers. The pattern of α7-2-nAChR expression was consistent with α7 staining in general, based on co-distribution of mAb319 and αBgt signals. However, α7-2-nAChRs clearly represent a distinct subset of α7 receptors. The α7-2-nAChR subtype was found throughout the cell-soma surface and was localized to a subpopulation of dendrites. Punctate staining characteristic of synaptic α7-2 targeting was observed at post-synaptic densities and intermittently at pre-synaptic locations. The α7-2 subunit was expressed on both GABAergic and non-GABAergic neurons. These studies reveal that receptors containing the α7-2 subunit constitute a subpopulation of α7-nAChRs and likely participate in cell-to-cell signaling in developing synapses of central neurons.

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KW - α-Bungarotoxin

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