Distribution and ontogeny of 1S,3R-1-aminocyclopentane-1,3-dicarboxylic acid-sensitive and quisqualate-insensitive [3H]glutamate binding sites in the rat brain

Rebecca A. Wright, John W. McDonald, Darryle D. Schoepp

Research output: Contribution to journalArticlepeer-review

Abstract

Displacement of [3H]glutamate by 1S,3R-1-aminocyclopentane-1,3- dicarboxylic acid and quisqualate (in the presence of saturating concentrations of ionotropic glutamate receptor agonists) was used to characterize optimal ionic conditions, distribution, and the ontogeny of glutamate receptor binding sites in rat brain. Using rat forebrain membranes or receptor autoradiography, optimal 1S,3R-1-aminocyclopentane-1,3- dicarboxylic acid-sensitive [3H]glutamate binding was found in the presence of 100 mM bromide ions and in the absence of calcium ions. Under these conditions, [3H]glutamate binding was relatively quisqualate insensitive. In regions of the neonatal (11-day-old) and adult rat brain, this [3H]glutamate binding was highest in forebrain (striatum, cerebral cortex, and hippocampus) and hypothalamus/midbrain but was lower in the cerebellum, olfactory bulb, and pons/medulla regions. 1S,3R-1-aminocyclopentane-1,3-dicarboxylic acid- sensitive and quisqualate-insensitive [3H]glutamate binding was present in the rat forebrain at 1 day of age and gradually increased more than twofold by day 50 (adult). Thus, in the presence of bromide ions and in the absence of calcium ions, [3H]glutamate labels a subpopulation of metabotropic glutamate receptors that are sensitive to 1S,3R-1-aminocyclopentane-1,3- dicarboxylic acid but insensitive to quisqualate. Expression of [3H]glutamate binding under these conditions was both regionally and developmentally regulated in rat brain, suggesting that [3H]glutamate is labeling a distinct population of metabotropic glutamate receptors.

Original languageEnglish (US)
Pages (from-to)938-945
Number of pages8
JournalJournal of Neurochemistry
Volume63
Issue number3
DOIs
StatePublished - Sep 1994

Keywords

  • 1S,3R-1-Aminocyclopentane-1,3- dicarboxylic acid
  • Autoradiography
  • Bromide
  • Excitatory amino acid
  • Glutamate
  • Metabotropic glutamate receptors
  • Quisqualate
  • Receptor binding

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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