Distinguishing characteristics of a new neuroblastoma cell line

James T. Casper, Jeffrey M. Trent, Joseph Harb, Vicki Piaskowski, Marilyn Helmsworth, Jeanne Finlan, Daniel D. Von Hoff

Research output: Contribution to journalArticle

Abstract

The characteristics of a new neuroblastoma cell line (MC-NB-1) established from the bone marrow of a 2-year-old male are described. Morphologically, the cells appear as flattened and epithelial-like or as small and spherical. Electron microscopy demonstrated microtubules and dense core secretory granules. The doubling time was approximately 35 hr. Isoenzyme patterns and catecholamine secretion indicated a human line of neuronal origin. The soft agar tumor colony forming system demonstrated drug resistance in vitro comparable to in vivo nonresponsiveness. The stemline karyotype of MC-NB-1 is 44,Y,del(1) (p22:), -4, -7, +del(7)(q22:), -16, +t(7;16)(16pter→16q24::7q22→7q32), -17. Additionally, double-minute bodies were observed. However, no evidence of homogeneous staining regions (HSRs) were detected.

Original languageEnglish (US)
Pages (from-to)177-186
Number of pages10
JournalCancer Genetics and Cytogenetics
Volume10
Issue number2
DOIs
StatePublished - Oct 1983

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Casper, J. T., Trent, J. M., Harb, J., Piaskowski, V., Helmsworth, M., Finlan, J., & Von Hoff, D. D. (1983). Distinguishing characteristics of a new neuroblastoma cell line. Cancer Genetics and Cytogenetics, 10(2), 177-186. https://doi.org/10.1016/0165-4608(83)90122-X