Distinction of deep versus superficial clinical and nonclinical isolates of Trichosporon beigelii by isoenzymes and restriction fragment length polymorphisms of rDNA generated by polymerase chain reaction

B. J. Kemker, P. F. Lehmann, J. W. Lee, T. J. Walsh

Research output: Contribution to journalArticle

Abstract

Fifteen clinical and environmental strains of Trichosporon beigelli were analyzed for similarities by using morphological features, biochemical profiles based on carbon compound assimilation and uric acid utilization, isoenzyme electrophoresis, and restriction fragment length polymorphisms of a segment of genes coding for rRNA expanded with the polymerase chain reaction. The findings suggest that strains that cause invasive disease are distinct from the superficial and the nonclinical isolates and that isolates from the skin and mucosae represent a number of different organisms, including some environmental forms. The study shows that T. beigelii is a complex of genetically distinct organisms and that more than one type is found in clinical samples.

Original languageEnglish (US)
Pages (from-to)1677-1683
Number of pages7
JournalJournal of clinical microbiology
Volume29
Issue number8
DOIs
StatePublished - 1991

ASJC Scopus subject areas

  • Microbiology (medical)

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