Distinct recognition of antibodies to centromere proteins in primary Sjögren's syndrome compared with limited scleroderma

Research output: Contribution to journalArticle

Abstract

Background: Anticentromere antibodies are characteristically observed in scleroderma but have recently been reported in other autoimmune rheumatic disorders, including Sjögren's syndrome. It is not known whether distinct centromere proteins (CENP) are targeted in primary Sjögren's syndrome (pSS) and scleroderma. Objective: To determine whether antibodies to CENP-B and CENP-C are present in these two disorders. Methods: Sera from 45 patients with pSS and 33 with limited scleroderma were studied. All patients met classification criteria for pSS and scleroderma, respectively. Sera were used to immunoprecipitate in vitro translated CENP-B and CENP-C. The proportions recognising CENP-B or CENP-C were compared. Results: 10 of 45 patients (22%) with pSS and 18 of 33 (55%) with scleroderma had antibodies recognising CENPs (p = 0.004). Seven of 10 (70%) CENP positive patients with pSS recognised CENP-C alone, compared with one of 18 (6%) CENP positive patients with scleroderma (odds ratio (OR) = 40 (95% confidence interval (CI), 3.5 to 450) (p = 0.003). In contrast, the majority (15 of 18 (83%)) of CENP positive scleroderma sera recognised both CENP-B and CENP-C, compared with none of 10 pSS sera (OR = 93 (95% CI, 4.4 to 1979) (p = 0.0001). Conclusions: The pattern of CENP recognition differs markedly in pSS and limited scleroderma. While patients with pSS predominantly recognise CENP-C alone, dual recognition of CENP-B and CENP-C is most frequent in scleroderma. These findings suggest that obtaining antibodies to specific centromere antigens is useful diagnostically, and imply that distinct mechanisms underlie the unique patterns of centromere autoreactivity in pSS and scleroderma.

Original languageEnglish (US)
Pages (from-to)1028-1032
Number of pages5
JournalAnnals of the Rheumatic Diseases
Volume65
Issue number8
DOIs
StatePublished - Aug 2006

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Limited Scleroderma
Centromere Protein B
Centromere
Antibodies
Proteins
Serum
Odds Ratio
centromere protein C
Confidence Intervals
Antigens

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

@article{f117fab8783247c69d6966cf13d5392b,
title = "Distinct recognition of antibodies to centromere proteins in primary Sj{\"o}gren's syndrome compared with limited scleroderma",
abstract = "Background: Anticentromere antibodies are characteristically observed in scleroderma but have recently been reported in other autoimmune rheumatic disorders, including Sj{\"o}gren's syndrome. It is not known whether distinct centromere proteins (CENP) are targeted in primary Sj{\"o}gren's syndrome (pSS) and scleroderma. Objective: To determine whether antibodies to CENP-B and CENP-C are present in these two disorders. Methods: Sera from 45 patients with pSS and 33 with limited scleroderma were studied. All patients met classification criteria for pSS and scleroderma, respectively. Sera were used to immunoprecipitate in vitro translated CENP-B and CENP-C. The proportions recognising CENP-B or CENP-C were compared. Results: 10 of 45 patients (22{\%}) with pSS and 18 of 33 (55{\%}) with scleroderma had antibodies recognising CENPs (p = 0.004). Seven of 10 (70{\%}) CENP positive patients with pSS recognised CENP-C alone, compared with one of 18 (6{\%}) CENP positive patients with scleroderma (odds ratio (OR) = 40 (95{\%} confidence interval (CI), 3.5 to 450) (p = 0.003). In contrast, the majority (15 of 18 (83{\%})) of CENP positive scleroderma sera recognised both CENP-B and CENP-C, compared with none of 10 pSS sera (OR = 93 (95{\%} CI, 4.4 to 1979) (p = 0.0001). Conclusions: The pattern of CENP recognition differs markedly in pSS and limited scleroderma. While patients with pSS predominantly recognise CENP-C alone, dual recognition of CENP-B and CENP-C is most frequent in scleroderma. These findings suggest that obtaining antibodies to specific centromere antigens is useful diagnostically, and imply that distinct mechanisms underlie the unique patterns of centromere autoreactivity in pSS and scleroderma.",
author = "Allan Gelber and Pillemer, {S. R.} and Baum, {B. J.} and Fredrick Wigley and Laura Hummers and S. Morris and Antony Rosen and {Casciola Rosen}, {Livia A}",
year = "2006",
month = "8",
doi = "10.1136/ard.2005.046003",
language = "English (US)",
volume = "65",
pages = "1028--1032",
journal = "Annals of the Rheumatic Diseases",
issn = "0003-4967",
publisher = "BMJ Publishing Group",
number = "8",

}

TY - JOUR

T1 - Distinct recognition of antibodies to centromere proteins in primary Sjögren's syndrome compared with limited scleroderma

AU - Gelber, Allan

AU - Pillemer, S. R.

AU - Baum, B. J.

AU - Wigley, Fredrick

AU - Hummers, Laura

AU - Morris, S.

AU - Rosen, Antony

AU - Casciola Rosen, Livia A

PY - 2006/8

Y1 - 2006/8

N2 - Background: Anticentromere antibodies are characteristically observed in scleroderma but have recently been reported in other autoimmune rheumatic disorders, including Sjögren's syndrome. It is not known whether distinct centromere proteins (CENP) are targeted in primary Sjögren's syndrome (pSS) and scleroderma. Objective: To determine whether antibodies to CENP-B and CENP-C are present in these two disorders. Methods: Sera from 45 patients with pSS and 33 with limited scleroderma were studied. All patients met classification criteria for pSS and scleroderma, respectively. Sera were used to immunoprecipitate in vitro translated CENP-B and CENP-C. The proportions recognising CENP-B or CENP-C were compared. Results: 10 of 45 patients (22%) with pSS and 18 of 33 (55%) with scleroderma had antibodies recognising CENPs (p = 0.004). Seven of 10 (70%) CENP positive patients with pSS recognised CENP-C alone, compared with one of 18 (6%) CENP positive patients with scleroderma (odds ratio (OR) = 40 (95% confidence interval (CI), 3.5 to 450) (p = 0.003). In contrast, the majority (15 of 18 (83%)) of CENP positive scleroderma sera recognised both CENP-B and CENP-C, compared with none of 10 pSS sera (OR = 93 (95% CI, 4.4 to 1979) (p = 0.0001). Conclusions: The pattern of CENP recognition differs markedly in pSS and limited scleroderma. While patients with pSS predominantly recognise CENP-C alone, dual recognition of CENP-B and CENP-C is most frequent in scleroderma. These findings suggest that obtaining antibodies to specific centromere antigens is useful diagnostically, and imply that distinct mechanisms underlie the unique patterns of centromere autoreactivity in pSS and scleroderma.

AB - Background: Anticentromere antibodies are characteristically observed in scleroderma but have recently been reported in other autoimmune rheumatic disorders, including Sjögren's syndrome. It is not known whether distinct centromere proteins (CENP) are targeted in primary Sjögren's syndrome (pSS) and scleroderma. Objective: To determine whether antibodies to CENP-B and CENP-C are present in these two disorders. Methods: Sera from 45 patients with pSS and 33 with limited scleroderma were studied. All patients met classification criteria for pSS and scleroderma, respectively. Sera were used to immunoprecipitate in vitro translated CENP-B and CENP-C. The proportions recognising CENP-B or CENP-C were compared. Results: 10 of 45 patients (22%) with pSS and 18 of 33 (55%) with scleroderma had antibodies recognising CENPs (p = 0.004). Seven of 10 (70%) CENP positive patients with pSS recognised CENP-C alone, compared with one of 18 (6%) CENP positive patients with scleroderma (odds ratio (OR) = 40 (95% confidence interval (CI), 3.5 to 450) (p = 0.003). In contrast, the majority (15 of 18 (83%)) of CENP positive scleroderma sera recognised both CENP-B and CENP-C, compared with none of 10 pSS sera (OR = 93 (95% CI, 4.4 to 1979) (p = 0.0001). Conclusions: The pattern of CENP recognition differs markedly in pSS and limited scleroderma. While patients with pSS predominantly recognise CENP-C alone, dual recognition of CENP-B and CENP-C is most frequent in scleroderma. These findings suggest that obtaining antibodies to specific centromere antigens is useful diagnostically, and imply that distinct mechanisms underlie the unique patterns of centromere autoreactivity in pSS and scleroderma.

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U2 - 10.1136/ard.2005.046003

DO - 10.1136/ard.2005.046003

M3 - Article

VL - 65

SP - 1028

EP - 1032

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JF - Annals of the Rheumatic Diseases

SN - 0003-4967

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