Distinct patterns of tryptophan maintenance in tissues during kynurenine pathway activation in simian immunodeficiency virus-infected macaques

Julia Drewes, Joshua D. Croteau, Erin N. Shirk, Elizabeth L. Engle, Mary Christine Zink, David Graham

Research output: Contribution to journalArticle

Abstract

Induction of the kynurenine pathway (KP) of tryptophan (TRP) catabolism has been proposed to contribute to T cell dysfunction during human/simian immunodeficiency virus (SIV) infection via depletion of local TRP levels and production of immunomodulatory KP metabolites. However, while changes in TRP and KP metabolites have been observed in plasma, their levels in lymphoid tissues and levels of enzymes downstream of indoleamine 2,3-dioxygenase (IDO1) have been relatively unexplored. We used our SIV-infected pigtailed macaque model to analyze longitudinal changes in KP metabolites and enzymes by gas chromatography/mass spectrometry and NanoString nCounter gene expression analysis, respectively, in spleen and blood compared to changes previously established in brain and CSF. We found that TRP levels were remarkably stable in tissue sites despite robust depletion in the circulating plasma and CSF. We also demonstrated that intracellular TRP reserves were maintained in cultured cells even in the presence of depleted extracellular TRP levels. Kynurenine (KYN),3-hydroxykynurenine, quinolinic acid, and the KP enzymes all displayed highly divergent patterns in the sites examined, though IDO1 expression always correlated with local KYN/TRP ratios. Finally, we demonstrated by fluorescence-activated cell sorting that myeloid dendritic cells and cells of monocytic lineage were the highest producers of IDO1 in chronically infected spleens. Overall, our study reveals insights into the tissue-specific regulation of KP enzymes and metabolites and, in particular, highlights the multiple mechanisms by which cells and tissues seek to prevent TRP starvation during inflammation.

Original languageEnglish (US)
Article number605
JournalFrontiers in Immunology
Volume7
Issue numberDEC
DOIs
StatePublished - 2016

Fingerprint

Kynurenine
Simian Immunodeficiency Virus
Macaca
Tryptophan
Maintenance
Enzymes
Spleen
Indoleamine-Pyrrole 2,3,-Dioxygenase
Quinolinic Acid
Lymphoid Tissue
Cell Lineage
Virus Diseases
Myeloid Cells
Starvation
Gas Chromatography-Mass Spectrometry
Dendritic Cells
Cultured Cells
Flow Cytometry
HIV
Inflammation

Keywords

  • HIV
  • IDO1
  • Immunosuppression
  • Kynurenine
  • SIV
  • Spleen
  • Tryptophan

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Distinct patterns of tryptophan maintenance in tissues during kynurenine pathway activation in simian immunodeficiency virus-infected macaques. / Drewes, Julia; Croteau, Joshua D.; Shirk, Erin N.; Engle, Elizabeth L.; Zink, Mary Christine; Graham, David.

In: Frontiers in Immunology, Vol. 7, No. DEC, 605, 2016.

Research output: Contribution to journalArticle

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