Distinct pathways of pathogenesis of intraductal oncocytic papillary neoplasms and intraductal papillary mucinous neoplasms of the pancreas

Olca Basturk, Sun M. Chung, Ralph H Hruban, N. Volkan Adsay, Gokce Askan, Christine Iacobuzio-Donahue, Serdar Balci, Sui Y. Zee, Bahar Memis, Jinru Shia, David S. Klimstra

Research output: Contribution to journalArticle

Abstract

Intraductal oncocytic papillary neoplasm (IOPN) of the pancreas is classified as a variant of intraductal papillary mucinous neoplasm (IPMN) in the WHO guidelines. However, the neoplastic cells of IOPNs are unique, with distinctive architecture/oncocytic cytoplasm. Although molecular/immunohistochemical features of other IPMN variants have been extensively studied, those of IOPNs have not been well characterized. Expression profile of antibodies associated with genetic alterations previously described for ductal adenocarcinomas (DAs) and IPMNs (SMAD4/β-catenin/p53/mesothelin/claudin-4) as well as antibodies to mucins and differentiation markers [MUC1/MUC2/MUC5AC/MUC6/CDX2/hepatocyte paraffin-1 (HepPar-1)] was investigated in 24 IOPNs and 22 IPMNs to assess the similarities/differences between these tumors. Expression of mesothelin and claudin-4 was dissimilar between these tumor types: A higher proportion of IOPNs labeled with mesothelin [21/24 (87.5 %) of IOPNs, 6/22 (27 %) of IPMNs, p < 0.001], while the reverse was true for claudin-4 [2/23 (9 %) of IOPNs, 9/22 (41 %) of IPMNs, p = 0.01]. The results of immunolabeling for SMAD4/β-catenin/p53 were similar in both: None of the cases showed SMAD4 loss in the intraductal components, and only 1/21 (5 %) of IOPNs and 2/22 (9 %) of IPMNs revealed abnormal β-catenin expression (p = 0.49). Nuclear p53 accumulation was seen mostly in architecturally complex/high-grade dysplasia areas in both. Immunolabeling for MUC proteins showed that almost all lesions expressed MUC5AC. Twelve of the 24 (50 %) IOPNs and 6/22 (27 %) of IPMNs (p = 0.11) labeled for MUC1, whereas 7/24 (29 %) of IOPNs and 10/22 (45 %) of IPMNs labeled for MUC2 (p = 0.25). MUC6 was expressed in 8/9 (89 %) of IOPNs (strong) and 6/21 (29 %) of IPMNs (weak) (p = 0.002). Fourteen of the 23 (61 %) IOPNs and 4/22 (18 %) of IPMNs labeled for HepPar-1 (p = 0.003). These results show that IOPNs have distinct immunoprofile and provide support for the proposition that IOPN is a distinct entity developing through a mechanism different from other pancreatic ductal neoplasms.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalVirchows Archiv
DOIs
StateAccepted/In press - Sep 3 2016

Fingerprint

Pancreatic Neoplasms
Claudin-4
Catenins
Neoplasms
Paraffin
Hepatocytes
Claudin-2
Antibodies
Differentiation Antigens
Mucins
Cytoplasm
Adenocarcinoma
Guidelines
mesothelin
Proteins

Keywords

  • Immunohistochemistry
  • IOPN
  • IPMN
  • Oncocytic
  • Pancreas

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Distinct pathways of pathogenesis of intraductal oncocytic papillary neoplasms and intraductal papillary mucinous neoplasms of the pancreas. / Basturk, Olca; Chung, Sun M.; Hruban, Ralph H; Adsay, N. Volkan; Askan, Gokce; Iacobuzio-Donahue, Christine; Balci, Serdar; Zee, Sui Y.; Memis, Bahar; Shia, Jinru; Klimstra, David S.

In: Virchows Archiv, 03.09.2016, p. 1-10.

Research output: Contribution to journalArticle

Basturk, O, Chung, SM, Hruban, RH, Adsay, NV, Askan, G, Iacobuzio-Donahue, C, Balci, S, Zee, SY, Memis, B, Shia, J & Klimstra, DS 2016, 'Distinct pathways of pathogenesis of intraductal oncocytic papillary neoplasms and intraductal papillary mucinous neoplasms of the pancreas', Virchows Archiv, pp. 1-10. https://doi.org/10.1007/s00428-016-2014-x
Basturk, Olca ; Chung, Sun M. ; Hruban, Ralph H ; Adsay, N. Volkan ; Askan, Gokce ; Iacobuzio-Donahue, Christine ; Balci, Serdar ; Zee, Sui Y. ; Memis, Bahar ; Shia, Jinru ; Klimstra, David S. / Distinct pathways of pathogenesis of intraductal oncocytic papillary neoplasms and intraductal papillary mucinous neoplasms of the pancreas. In: Virchows Archiv. 2016 ; pp. 1-10.
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abstract = "Intraductal oncocytic papillary neoplasm (IOPN) of the pancreas is classified as a variant of intraductal papillary mucinous neoplasm (IPMN) in the WHO guidelines. However, the neoplastic cells of IOPNs are unique, with distinctive architecture/oncocytic cytoplasm. Although molecular/immunohistochemical features of other IPMN variants have been extensively studied, those of IOPNs have not been well characterized. Expression profile of antibodies associated with genetic alterations previously described for ductal adenocarcinomas (DAs) and IPMNs (SMAD4/β-catenin/p53/mesothelin/claudin-4) as well as antibodies to mucins and differentiation markers [MUC1/MUC2/MUC5AC/MUC6/CDX2/hepatocyte paraffin-1 (HepPar-1)] was investigated in 24 IOPNs and 22 IPMNs to assess the similarities/differences between these tumors. Expression of mesothelin and claudin-4 was dissimilar between these tumor types: A higher proportion of IOPNs labeled with mesothelin [21/24 (87.5 {\%}) of IOPNs, 6/22 (27 {\%}) of IPMNs, p < 0.001], while the reverse was true for claudin-4 [2/23 (9 {\%}) of IOPNs, 9/22 (41 {\%}) of IPMNs, p = 0.01]. The results of immunolabeling for SMAD4/β-catenin/p53 were similar in both: None of the cases showed SMAD4 loss in the intraductal components, and only 1/21 (5 {\%}) of IOPNs and 2/22 (9 {\%}) of IPMNs revealed abnormal β-catenin expression (p = 0.49). Nuclear p53 accumulation was seen mostly in architecturally complex/high-grade dysplasia areas in both. Immunolabeling for MUC proteins showed that almost all lesions expressed MUC5AC. Twelve of the 24 (50 {\%}) IOPNs and 6/22 (27 {\%}) of IPMNs (p = 0.11) labeled for MUC1, whereas 7/24 (29 {\%}) of IOPNs and 10/22 (45 {\%}) of IPMNs labeled for MUC2 (p = 0.25). MUC6 was expressed in 8/9 (89 {\%}) of IOPNs (strong) and 6/21 (29 {\%}) of IPMNs (weak) (p = 0.002). Fourteen of the 23 (61 {\%}) IOPNs and 4/22 (18 {\%}) of IPMNs labeled for HepPar-1 (p = 0.003). These results show that IOPNs have distinct immunoprofile and provide support for the proposition that IOPN is a distinct entity developing through a mechanism different from other pancreatic ductal neoplasms.",
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AU - Chung, Sun M.

AU - Hruban, Ralph H

AU - Adsay, N. Volkan

AU - Askan, Gokce

AU - Iacobuzio-Donahue, Christine

AU - Balci, Serdar

AU - Zee, Sui Y.

AU - Memis, Bahar

AU - Shia, Jinru

AU - Klimstra, David S.

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N2 - Intraductal oncocytic papillary neoplasm (IOPN) of the pancreas is classified as a variant of intraductal papillary mucinous neoplasm (IPMN) in the WHO guidelines. However, the neoplastic cells of IOPNs are unique, with distinctive architecture/oncocytic cytoplasm. Although molecular/immunohistochemical features of other IPMN variants have been extensively studied, those of IOPNs have not been well characterized. Expression profile of antibodies associated with genetic alterations previously described for ductal adenocarcinomas (DAs) and IPMNs (SMAD4/β-catenin/p53/mesothelin/claudin-4) as well as antibodies to mucins and differentiation markers [MUC1/MUC2/MUC5AC/MUC6/CDX2/hepatocyte paraffin-1 (HepPar-1)] was investigated in 24 IOPNs and 22 IPMNs to assess the similarities/differences between these tumors. Expression of mesothelin and claudin-4 was dissimilar between these tumor types: A higher proportion of IOPNs labeled with mesothelin [21/24 (87.5 %) of IOPNs, 6/22 (27 %) of IPMNs, p < 0.001], while the reverse was true for claudin-4 [2/23 (9 %) of IOPNs, 9/22 (41 %) of IPMNs, p = 0.01]. The results of immunolabeling for SMAD4/β-catenin/p53 were similar in both: None of the cases showed SMAD4 loss in the intraductal components, and only 1/21 (5 %) of IOPNs and 2/22 (9 %) of IPMNs revealed abnormal β-catenin expression (p = 0.49). Nuclear p53 accumulation was seen mostly in architecturally complex/high-grade dysplasia areas in both. Immunolabeling for MUC proteins showed that almost all lesions expressed MUC5AC. Twelve of the 24 (50 %) IOPNs and 6/22 (27 %) of IPMNs (p = 0.11) labeled for MUC1, whereas 7/24 (29 %) of IOPNs and 10/22 (45 %) of IPMNs labeled for MUC2 (p = 0.25). MUC6 was expressed in 8/9 (89 %) of IOPNs (strong) and 6/21 (29 %) of IPMNs (weak) (p = 0.002). Fourteen of the 23 (61 %) IOPNs and 4/22 (18 %) of IPMNs labeled for HepPar-1 (p = 0.003). These results show that IOPNs have distinct immunoprofile and provide support for the proposition that IOPN is a distinct entity developing through a mechanism different from other pancreatic ductal neoplasms.

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