Distinct LIN-10 domains are required for its neuronal function, its epithelial function, and its synaptic localization

Doreen R. Glodowski, Tricia Wright, Keri Martinowich, Howard Chia Hao Chang, Douglas Beach, Christopher Rongo

Research output: Contribution to journalArticlepeer-review

Abstract

α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)-type glutamate receptors (AMPARs) mediate excitatory neurotransmission at neuronal synapses, and their regulated localization plays a role in synaptic plasticity. In Caenorhabditis elegans, the PDZ and PTB domain-containing protein LIN-10 is required both for the synaptic localization of the AMPAR subunit GLR-1 and for vulval fate induction in epithelia. Here, we examine the role that different LIN-10 domains play in GLR-1 localization. We find that an amino-terminal region of LIN-10 directs LIN-10 protein localization to the Golgi and to synaptic clusters. In addition, mutations in the carboxyl-terminal PDZ domains prevent LIN-10 from regulating GLR-1 localization in neurons but do not prevent LIN-10 from functioning in the vulval epithelia. A mutation in the amino terminus prevents the protein from functioning in the vulval epithelia but does not prevent it from functioning to regulate GLR-1 localization in neurons. Finally, we show that human Mint2 can substitute for LIN-10 to facilitate GLR-1 localization in neurons and that the Mint2 amino terminus is critical for this function. Together, our data suggest that LIN-10 uses distinct modular domains for its functions in neurons and epithelial cells and that during evolution its vertebrate ortholog Mint2 has retained the ability to direct AMPAR localization in neurons.

Original languageEnglish (US)
Pages (from-to)1417-1426
Number of pages10
JournalMolecular biology of the cell
Volume16
Issue number3
DOIs
StatePublished - Mar 2005

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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