Distinct high-profile methylated genes in colorectal cancer

Pooneh Mokarram, Krishan Kumar, Hassan Brim, Fakhraddin Naghibalhossaini, Mehdi Saberifiroozi, Mehdi Nouraie, Robert Green, Ed Lee, Duane T. Smoot, Hassan Ashktorab

Research output: Contribution to journalArticle

Abstract

Background: Mutations and promoters' methylation of a set of candidate cancer genes (CAN genes) are associated with progression of colorectal cancer (CRC). We hypothesized that these genes' promoters are inactivated through epigenetic silencing and may show a different profile in high-risk populations. We investigated the status of CAN gene methylation and CHD5 protein expression in African American CRC tissue microarrays (TMA) using immunohistochemical staining. Methodology/Principal Findings: The promoter methylation status of the CAN genes was studied by methylation-specific PCR (MSP) in 51 Iranians (a white population) and 51 African Americans (AA). Microsatellite instability (MSI) was analyzed as well. The differential frequency of methylation for each gene was tested by chi-square analysis between the two groups based on matched age and sex. CHD5 protein expression was evaluated in moderate to well differentiated and poorly differentiated carcinomas compared to matched normal tissue using TMA. In addition, the correlation between these epigenetic biomarkers and various clinicopathological factors, including, age, location, and stage of the disease were analyzed. Seventy-seven and 34% of tumors were distal in Iranian and African American patients, respectively. In both populations, the percentage of methylation was >65% for SYNE1, MMP2, APC2, GPNMB, EVL, PTPRD, and STARD8, whereas methylation was

Original languageEnglish (US)
Article numbere7012
JournalPLoS One
Volume4
Issue number9
DOIs
StatePublished - Sep 11 2009
Externally publishedYes

Fingerprint

Methylation
colorectal neoplasms
methylation
Colorectal Neoplasms
Genes
Neoplasm Genes
African Americans
genes
neoplasms
promoter regions
Tissue
Microarrays
Epigenomics
epigenetics
protein synthesis
Population
Microsatellite Instability
Age Factors
Biomarkers
Microsatellite Repeats

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Mokarram, P., Kumar, K., Brim, H., Naghibalhossaini, F., Saberifiroozi, M., Nouraie, M., ... Ashktorab, H. (2009). Distinct high-profile methylated genes in colorectal cancer. PLoS One, 4(9), [e7012]. https://doi.org/10.1371/journal.pone.0007012

Distinct high-profile methylated genes in colorectal cancer. / Mokarram, Pooneh; Kumar, Krishan; Brim, Hassan; Naghibalhossaini, Fakhraddin; Saberifiroozi, Mehdi; Nouraie, Mehdi; Green, Robert; Lee, Ed; Smoot, Duane T.; Ashktorab, Hassan.

In: PLoS One, Vol. 4, No. 9, e7012, 11.09.2009.

Research output: Contribution to journalArticle

Mokarram, P, Kumar, K, Brim, H, Naghibalhossaini, F, Saberifiroozi, M, Nouraie, M, Green, R, Lee, E, Smoot, DT & Ashktorab, H 2009, 'Distinct high-profile methylated genes in colorectal cancer', PLoS One, vol. 4, no. 9, e7012. https://doi.org/10.1371/journal.pone.0007012
Mokarram P, Kumar K, Brim H, Naghibalhossaini F, Saberifiroozi M, Nouraie M et al. Distinct high-profile methylated genes in colorectal cancer. PLoS One. 2009 Sep 11;4(9). e7012. https://doi.org/10.1371/journal.pone.0007012
Mokarram, Pooneh ; Kumar, Krishan ; Brim, Hassan ; Naghibalhossaini, Fakhraddin ; Saberifiroozi, Mehdi ; Nouraie, Mehdi ; Green, Robert ; Lee, Ed ; Smoot, Duane T. ; Ashktorab, Hassan. / Distinct high-profile methylated genes in colorectal cancer. In: PLoS One. 2009 ; Vol. 4, No. 9.
@article{c1bd9990d0a44f89a64c1a9c149d577c,
title = "Distinct high-profile methylated genes in colorectal cancer",
abstract = "Background: Mutations and promoters' methylation of a set of candidate cancer genes (CAN genes) are associated with progression of colorectal cancer (CRC). We hypothesized that these genes' promoters are inactivated through epigenetic silencing and may show a different profile in high-risk populations. We investigated the status of CAN gene methylation and CHD5 protein expression in African American CRC tissue microarrays (TMA) using immunohistochemical staining. Methodology/Principal Findings: The promoter methylation status of the CAN genes was studied by methylation-specific PCR (MSP) in 51 Iranians (a white population) and 51 African Americans (AA). Microsatellite instability (MSI) was analyzed as well. The differential frequency of methylation for each gene was tested by chi-square analysis between the two groups based on matched age and sex. CHD5 protein expression was evaluated in moderate to well differentiated and poorly differentiated carcinomas compared to matched normal tissue using TMA. In addition, the correlation between these epigenetic biomarkers and various clinicopathological factors, including, age, location, and stage of the disease were analyzed. Seventy-seven and 34{\%} of tumors were distal in Iranian and African American patients, respectively. In both populations, the percentage of methylation was >65{\%} for SYNE1, MMP2, APC2, GPNMB, EVL, PTPRD, and STARD8, whereas methylation was",
author = "Pooneh Mokarram and Krishan Kumar and Hassan Brim and Fakhraddin Naghibalhossaini and Mehdi Saberifiroozi and Mehdi Nouraie and Robert Green and Ed Lee and Smoot, {Duane T.} and Hassan Ashktorab",
year = "2009",
month = "9",
day = "11",
doi = "10.1371/journal.pone.0007012",
language = "English (US)",
volume = "4",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "9",

}

TY - JOUR

T1 - Distinct high-profile methylated genes in colorectal cancer

AU - Mokarram, Pooneh

AU - Kumar, Krishan

AU - Brim, Hassan

AU - Naghibalhossaini, Fakhraddin

AU - Saberifiroozi, Mehdi

AU - Nouraie, Mehdi

AU - Green, Robert

AU - Lee, Ed

AU - Smoot, Duane T.

AU - Ashktorab, Hassan

PY - 2009/9/11

Y1 - 2009/9/11

N2 - Background: Mutations and promoters' methylation of a set of candidate cancer genes (CAN genes) are associated with progression of colorectal cancer (CRC). We hypothesized that these genes' promoters are inactivated through epigenetic silencing and may show a different profile in high-risk populations. We investigated the status of CAN gene methylation and CHD5 protein expression in African American CRC tissue microarrays (TMA) using immunohistochemical staining. Methodology/Principal Findings: The promoter methylation status of the CAN genes was studied by methylation-specific PCR (MSP) in 51 Iranians (a white population) and 51 African Americans (AA). Microsatellite instability (MSI) was analyzed as well. The differential frequency of methylation for each gene was tested by chi-square analysis between the two groups based on matched age and sex. CHD5 protein expression was evaluated in moderate to well differentiated and poorly differentiated carcinomas compared to matched normal tissue using TMA. In addition, the correlation between these epigenetic biomarkers and various clinicopathological factors, including, age, location, and stage of the disease were analyzed. Seventy-seven and 34% of tumors were distal in Iranian and African American patients, respectively. In both populations, the percentage of methylation was >65% for SYNE1, MMP2, APC2, GPNMB, EVL, PTPRD, and STARD8, whereas methylation was

AB - Background: Mutations and promoters' methylation of a set of candidate cancer genes (CAN genes) are associated with progression of colorectal cancer (CRC). We hypothesized that these genes' promoters are inactivated through epigenetic silencing and may show a different profile in high-risk populations. We investigated the status of CAN gene methylation and CHD5 protein expression in African American CRC tissue microarrays (TMA) using immunohistochemical staining. Methodology/Principal Findings: The promoter methylation status of the CAN genes was studied by methylation-specific PCR (MSP) in 51 Iranians (a white population) and 51 African Americans (AA). Microsatellite instability (MSI) was analyzed as well. The differential frequency of methylation for each gene was tested by chi-square analysis between the two groups based on matched age and sex. CHD5 protein expression was evaluated in moderate to well differentiated and poorly differentiated carcinomas compared to matched normal tissue using TMA. In addition, the correlation between these epigenetic biomarkers and various clinicopathological factors, including, age, location, and stage of the disease were analyzed. Seventy-seven and 34% of tumors were distal in Iranian and African American patients, respectively. In both populations, the percentage of methylation was >65% for SYNE1, MMP2, APC2, GPNMB, EVL, PTPRD, and STARD8, whereas methylation was

UR - http://www.scopus.com/inward/record.url?scp=70349183918&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349183918&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0007012

DO - 10.1371/journal.pone.0007012

M3 - Article

C2 - 19750230

AN - SCOPUS:70349183918

VL - 4

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 9

M1 - e7012

ER -