Distinct factors regulate the murine RAG-2 promoter in B- and T-cell lines

Josh Lauring, Mark S. Schlissel

Research output: Contribution to journalArticlepeer-review

Abstract

The recombination activating genes RAG-1 and RAG-2 are expressed in a lymphoid-cell-specific and developmentally regulated fashion. To understand the transcriptional basis for this regulation, we have cloned and characterized the murine RAG-2 promoter. The promoter was lymphoid cell specific, showing activity in various B- and T-cell lines but little activity in nonlymphoid cells. To our surprise, however, the promoter was regulated differently in B and T cells. Using nuclear extracts from B-cell lines, we found that the B-cell-specific transcription factor BSAP (Pax-5) could bind to a conserved sequence critical for promoter activity. BSAP activated the promoter in transfected cells, and the BSAP site was occupied in a tissue- specific manner in vivo. An overlapping DNA sequence binding to a distinct factor was necessary for promoter activity in T cells. Full promoter activity in T cells was also dependent on a more distal DNA sequence whose disruption had no effect on B-cell activity. The unexpected finding that a B-cell- specific factor regulates the RAG-2 promoter may explain some of the recently observed differences in the regulation of RAG transcription between B and T cells.

Original languageEnglish (US)
Pages (from-to)2601-2612
Number of pages12
JournalMolecular and cellular biology
Volume19
Issue number4
DOIs
StatePublished - Apr 1999

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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