Distinct expression profiles of transcriptional coactivators for thyroid hormone receptors during Xenopus laevis metamorphosis

Bindu D. Paul, Yun Bo Shi

Research output: Contribution to journalArticlepeer-review

Abstract

The biological effects of thyroid hormone (T3) are mediated by the thyroid hormone receptor (TR). Amphibian metamorphosis is one of the most dramatic processes that are dependent on T3. T3 regulates a series of orchestrated developmental changes, which ultimately result in the conversion of an aquatic herbivorous tadpole to a terrestrial carnivorous frog. T3 is presumed to bind to TRs, which in turn recruit coactivators, leading to gene activation. The best-studied coactivators belong to the p160 or SRC family. Members of this family include SRC1/ NCoA-1, SRC2/TIF2/GRIP1, and SRC3/pCIP/ACTR/AIB-1/RAC-3/TRAM-1. These SRCs interact directly with liganded TR and function as adapter molecules to recruit other coactivators such as p300/CBP. Here, we studied the expression patterns of these coactivators during various stages of development. Amongst the coactivators cloned in Xenopus laevis, SRC3 was found to be dramatically upregulated during natural and T 3-induced metamorphosis, and SRC2 and p300 are expressed throughout postembryonic development with little change in their expression levels. These results support the view that these coactivators participate in gene regulation by TR during metamorphosis.

Original languageEnglish (US)
Pages (from-to)459-464
Number of pages6
JournalCell Research
Volume13
Issue number6
DOIs
StatePublished - Dec 2003
Externally publishedYes

Keywords

  • Histone acetylation
  • Metamorphosis
  • Thyroid hormone receptor
  • Transcription coactivators
  • Xenopus laevis

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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