Distinct energy requirements for human memory CD4 T-cell homeostatic functions

Dennis D. Taub, Charles S. Hesdorffer, Luigi Ferrucci, Karen Madara, Janice B. Schwartz, Edward J. Goetzl

Research output: Contribution to journalArticle

Abstract

Differentiation and activation of CD4 memory T cells (Tmem cells) require energy from different sources, but little is known about energy sources for maintenance and surveillance activities of unactivated T mem cells. Mitochondrial fatty acid oxidation (FAO) in human unactivated CD4 Tmem cells was significantly enhanced by inhibition of glycolysis, with respective means of 1.7- and 4.5-fold for subjects 65 yr, and by stimulation of AMP-activated protein kinase, with respective means of 1.3- and 5.2-fold. However, CCL19 and sphingosine 1-phosphate (S1P), which control homeostatic lymphoid trafficking of unactivated Tmem cells, altered FAO and glycolysis only minimally or not at all. Inhibition of CD4 Tmem-cell basal FAO, but not basal glycolysis, significantly suppressed CCL19- and S1P-mediated adherence to collagen by >50 and 20%, respectively, and chemotaxis by >20 and 50%. Apoptosis of unactivated Tmem cells induced by IL-2 deprivation or CCL19 was increased significantly by >150 and 70%, respectively, with inhibition of FAO and by >110 and 30% with inhibition of glycolysis. Anti-TCR antibody activation of Tmem cells increased their chemotaxis to CCL5, which was dependent predominantly on glycolysis rather than FAO. The sources supplying energy for diverse functions of unactivated Tmem cells differ from that required for function after immune activation.

Original languageEnglish (US)
Pages (from-to)342-349
Number of pages8
JournalFASEB Journal
Volume27
Issue number1
DOIs
StatePublished - Jan 2013
Externally publishedYes

Fingerprint

T-cells
Fatty Acids
Glycolysis
T-Lymphocytes
Data storage equipment
Oxidation
Chemical activation
Chemotaxis
AMP-Activated Protein Kinases
Interleukin-2
Collagen
Apoptosis
Antibodies
Anti-Idiotypic Antibodies
Maintenance
Inhibition (Psychology)

Keywords

  • Adherence
  • Apoptosis
  • Chemotaxis
  • Fatty acid oxidation
  • Glycolysis
  • Immunosenescence

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology

Cite this

Taub, D. D., Hesdorffer, C. S., Ferrucci, L., Madara, K., Schwartz, J. B., & Goetzl, E. J. (2013). Distinct energy requirements for human memory CD4 T-cell homeostatic functions. FASEB Journal, 27(1), 342-349. https://doi.org/10.1096/fj.12-217620

Distinct energy requirements for human memory CD4 T-cell homeostatic functions. / Taub, Dennis D.; Hesdorffer, Charles S.; Ferrucci, Luigi; Madara, Karen; Schwartz, Janice B.; Goetzl, Edward J.

In: FASEB Journal, Vol. 27, No. 1, 01.2013, p. 342-349.

Research output: Contribution to journalArticle

Taub, DD, Hesdorffer, CS, Ferrucci, L, Madara, K, Schwartz, JB & Goetzl, EJ 2013, 'Distinct energy requirements for human memory CD4 T-cell homeostatic functions', FASEB Journal, vol. 27, no. 1, pp. 342-349. https://doi.org/10.1096/fj.12-217620
Taub DD, Hesdorffer CS, Ferrucci L, Madara K, Schwartz JB, Goetzl EJ. Distinct energy requirements for human memory CD4 T-cell homeostatic functions. FASEB Journal. 2013 Jan;27(1):342-349. https://doi.org/10.1096/fj.12-217620
Taub, Dennis D. ; Hesdorffer, Charles S. ; Ferrucci, Luigi ; Madara, Karen ; Schwartz, Janice B. ; Goetzl, Edward J. / Distinct energy requirements for human memory CD4 T-cell homeostatic functions. In: FASEB Journal. 2013 ; Vol. 27, No. 1. pp. 342-349.
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