Dissociation between vascular endothelial growth factor receptor-2 and blood vessel density in the caudate nucleus after chronic hydrocephalus

Abhishek Deshpande, Stephen M. Dombrowski, Anna Leichliter, Natalie Krajcir, Nicholas Zingales, Masahiro Inoue, Soren Schenk, Kiyotaka Fukamachi, Mark Luciano

Research output: Contribution to journalArticle

Abstract

Chronic hydrocephalus (CH) is characterized by the presence of ventricular enlargement, decreased cerebral blood flow (CBF), and brain tissue oxygen delivery. Although the underlying pathophysiological role of vascular endothelial growth factor (VEGF) is not clear, ischemic-hypoxic events in CH are known to trigger its release. Previously, we have shown increased VEGF receptor-2 (VEGFR-2) and blood vessel density (BVd) in the hippocampus after CH. We investigated changes in neuronal and glial VEGFR-2 density and BVd in the caudate nucleus in an experimental model of CH. Animals with CH were divided into short term (ST, 2 to 4 weeks) and long term (LT, 12 to 16 weeks) and were compared with surgical controls (SCs, 12 to 16 weeks). The cellular and BVds were estimated using immunohistochemical and stereological counting methods. Overall, percentage (%)VEGFR-2 neurons were approximately two times greater in CH (ST, LT) than in SC. By comparison, glial cell %VEGFR-2 was greater by 10% to 17% in ST and 4% to 11% lower in LT compared with that in SC. Blood vessel density was significantly lower in CH than in SC in the superficial caudate. Changes in cerebrospinal fluid ventricular volume and pressure, as well as in CBF did not correlate with either VEGFR-2 or BVd. These observed findings suggest that destructive forces may outweigh angiogenic forces and possibly show a disassociation between VEGFR-2 and BV expressions.

Original languageEnglish (US)
Pages (from-to)1806-1815
Number of pages10
JournalJournal of Cerebral Blood Flow and Metabolism
Volume29
Issue number11
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Vascular Endothelial Growth Factor Receptor-2
Caudate Nucleus
Hydrocephalus
Blood Vessels
Vascular Endothelial Growth Factor Receptor
Cerebrovascular Circulation
Neuroglia
Ventricular Pressure
Vascular Endothelial Growth Factor A
Cerebrospinal Fluid
Hippocampus
Theoretical Models
Oxygen
Neurons
Brain

Keywords

  • Angiogenesis
  • Canine
  • Cerebrovascular
  • Hypoxia
  • Ischemia

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Neurology

Cite this

Dissociation between vascular endothelial growth factor receptor-2 and blood vessel density in the caudate nucleus after chronic hydrocephalus. / Deshpande, Abhishek; Dombrowski, Stephen M.; Leichliter, Anna; Krajcir, Natalie; Zingales, Nicholas; Inoue, Masahiro; Schenk, Soren; Fukamachi, Kiyotaka; Luciano, Mark.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 29, No. 11, 2009, p. 1806-1815.

Research output: Contribution to journalArticle

Deshpande, Abhishek ; Dombrowski, Stephen M. ; Leichliter, Anna ; Krajcir, Natalie ; Zingales, Nicholas ; Inoue, Masahiro ; Schenk, Soren ; Fukamachi, Kiyotaka ; Luciano, Mark. / Dissociation between vascular endothelial growth factor receptor-2 and blood vessel density in the caudate nucleus after chronic hydrocephalus. In: Journal of Cerebral Blood Flow and Metabolism. 2009 ; Vol. 29, No. 11. pp. 1806-1815.
@article{719352b9cf174b728f469335b1d70408,
title = "Dissociation between vascular endothelial growth factor receptor-2 and blood vessel density in the caudate nucleus after chronic hydrocephalus",
abstract = "Chronic hydrocephalus (CH) is characterized by the presence of ventricular enlargement, decreased cerebral blood flow (CBF), and brain tissue oxygen delivery. Although the underlying pathophysiological role of vascular endothelial growth factor (VEGF) is not clear, ischemic-hypoxic events in CH are known to trigger its release. Previously, we have shown increased VEGF receptor-2 (VEGFR-2) and blood vessel density (BVd) in the hippocampus after CH. We investigated changes in neuronal and glial VEGFR-2 density and BVd in the caudate nucleus in an experimental model of CH. Animals with CH were divided into short term (ST, 2 to 4 weeks) and long term (LT, 12 to 16 weeks) and were compared with surgical controls (SCs, 12 to 16 weeks). The cellular and BVds were estimated using immunohistochemical and stereological counting methods. Overall, percentage ({\%})VEGFR-2 neurons were approximately two times greater in CH (ST, LT) than in SC. By comparison, glial cell {\%}VEGFR-2 was greater by 10{\%} to 17{\%} in ST and 4{\%} to 11{\%} lower in LT compared with that in SC. Blood vessel density was significantly lower in CH than in SC in the superficial caudate. Changes in cerebrospinal fluid ventricular volume and pressure, as well as in CBF did not correlate with either VEGFR-2 or BVd. These observed findings suggest that destructive forces may outweigh angiogenic forces and possibly show a disassociation between VEGFR-2 and BV expressions.",
keywords = "Angiogenesis, Canine, Cerebrovascular, Hypoxia, Ischemia",
author = "Abhishek Deshpande and Dombrowski, {Stephen M.} and Anna Leichliter and Natalie Krajcir and Nicholas Zingales and Masahiro Inoue and Soren Schenk and Kiyotaka Fukamachi and Mark Luciano",
year = "2009",
doi = "10.1038/jcbfm.2009.98",
language = "English (US)",
volume = "29",
pages = "1806--1815",
journal = "Journal of Cerebral Blood Flow and Metabolism",
issn = "0271-678X",
publisher = "Nature Publishing Group",
number = "11",

}

TY - JOUR

T1 - Dissociation between vascular endothelial growth factor receptor-2 and blood vessel density in the caudate nucleus after chronic hydrocephalus

AU - Deshpande, Abhishek

AU - Dombrowski, Stephen M.

AU - Leichliter, Anna

AU - Krajcir, Natalie

AU - Zingales, Nicholas

AU - Inoue, Masahiro

AU - Schenk, Soren

AU - Fukamachi, Kiyotaka

AU - Luciano, Mark

PY - 2009

Y1 - 2009

N2 - Chronic hydrocephalus (CH) is characterized by the presence of ventricular enlargement, decreased cerebral blood flow (CBF), and brain tissue oxygen delivery. Although the underlying pathophysiological role of vascular endothelial growth factor (VEGF) is not clear, ischemic-hypoxic events in CH are known to trigger its release. Previously, we have shown increased VEGF receptor-2 (VEGFR-2) and blood vessel density (BVd) in the hippocampus after CH. We investigated changes in neuronal and glial VEGFR-2 density and BVd in the caudate nucleus in an experimental model of CH. Animals with CH were divided into short term (ST, 2 to 4 weeks) and long term (LT, 12 to 16 weeks) and were compared with surgical controls (SCs, 12 to 16 weeks). The cellular and BVds were estimated using immunohistochemical and stereological counting methods. Overall, percentage (%)VEGFR-2 neurons were approximately two times greater in CH (ST, LT) than in SC. By comparison, glial cell %VEGFR-2 was greater by 10% to 17% in ST and 4% to 11% lower in LT compared with that in SC. Blood vessel density was significantly lower in CH than in SC in the superficial caudate. Changes in cerebrospinal fluid ventricular volume and pressure, as well as in CBF did not correlate with either VEGFR-2 or BVd. These observed findings suggest that destructive forces may outweigh angiogenic forces and possibly show a disassociation between VEGFR-2 and BV expressions.

AB - Chronic hydrocephalus (CH) is characterized by the presence of ventricular enlargement, decreased cerebral blood flow (CBF), and brain tissue oxygen delivery. Although the underlying pathophysiological role of vascular endothelial growth factor (VEGF) is not clear, ischemic-hypoxic events in CH are known to trigger its release. Previously, we have shown increased VEGF receptor-2 (VEGFR-2) and blood vessel density (BVd) in the hippocampus after CH. We investigated changes in neuronal and glial VEGFR-2 density and BVd in the caudate nucleus in an experimental model of CH. Animals with CH were divided into short term (ST, 2 to 4 weeks) and long term (LT, 12 to 16 weeks) and were compared with surgical controls (SCs, 12 to 16 weeks). The cellular and BVds were estimated using immunohistochemical and stereological counting methods. Overall, percentage (%)VEGFR-2 neurons were approximately two times greater in CH (ST, LT) than in SC. By comparison, glial cell %VEGFR-2 was greater by 10% to 17% in ST and 4% to 11% lower in LT compared with that in SC. Blood vessel density was significantly lower in CH than in SC in the superficial caudate. Changes in cerebrospinal fluid ventricular volume and pressure, as well as in CBF did not correlate with either VEGFR-2 or BVd. These observed findings suggest that destructive forces may outweigh angiogenic forces and possibly show a disassociation between VEGFR-2 and BV expressions.

KW - Angiogenesis

KW - Canine

KW - Cerebrovascular

KW - Hypoxia

KW - Ischemia

UR - http://www.scopus.com/inward/record.url?scp=71949097184&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71949097184&partnerID=8YFLogxK

U2 - 10.1038/jcbfm.2009.98

DO - 10.1038/jcbfm.2009.98

M3 - Article

C2 - 19675561

AN - SCOPUS:71949097184

VL - 29

SP - 1806

EP - 1815

JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

SN - 0271-678X

IS - 11

ER -