Dissecting independent channel and scaffolding roles of the Drosophila transient receptor potential channel

Tao Wang, Yuchen Jiao, Craig Montell

Research output: Contribution to journalArticle

Abstract

Drosophila transient receptor potential (TRP) serves dual roles as a cation channel and as a molecular anchor for the PDZ protein, INAD (inactivation no afterpotential D). Null mutations in trp cause impairment of visual transduction, mislocalization of INAD, and retinal degeneration. However, the impact of specifically altering TRP channel function is not known because existing loss-of-function alleles greatly reduce protein expression. In the current study we describe the isolation of a set of new trp alleles, including trp14 with an amino acid substitution juxtaposed to the TRP domain. The trp14 flies stably express TRP and display normal molecular anchoring, but defective channel function. Elimination of the anchoring function alone in trpΔ1272, had minor effects on retinal morphology whereas disruption of channel function caused profound light-induced cell death. This retinal degeneration was greatly suppressed by elimination of the Na +/Ca2+ exchanger, CalX, indicating that the cell death was due primarily to deficient Ca2+ entry rather than disruption of the TRP-anchoring function.

Original languageEnglish (US)
Pages (from-to)685-694
Number of pages10
JournalJournal of Cell Biology
Volume171
Issue number4
DOIs
Publication statusPublished - Nov 21 2005

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ASJC Scopus subject areas

  • Cell Biology

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