Disruptions in feeding and body weight control in gastrin-releasing peptide receptor deficient mice

Ellen Elizabeth Ladenheim, L. L. Hampton, A. C. Whitney, W. O. White, J. F. Battey, Timothy H Moran

Research output: Contribution to journalArticle

Abstract

Bombesin (BN) interacts with two mammalian receptor subtypes termed gastrin-releasing peptide (GRP)-preferring (GRP-R) and neuromedin B (NMB)-preferring (NMB-R) that may mediate the satiety action of BN. We examined the feeding behavior of mice that were deficient in the GRP-R (GRP-R KO) to assess the overall contribution of this receptor subtype in the feeding actions of BN-related peptides. GRP-R KO mice failed to suppress glucose intake in response to systemically administered BN and GRP18-27, whereas both peptides elicited a potent reduction of intake in wild-type (WT) mice. Neither GRP-R KO nor WT mice suppressed glucose intake following NMB administration. Unlike the impaired responses to BN-like peptides, the feeding inhibitory action of cholecystokinin was enhanced in GRP-R KO mice. Consistent with behavioral results, GRP-R KO mice also exhibited a reduction in c-Fos immunoreactivity in the nucleus of the solitary tract (NTS) and paraventricular nucleus (PVN) following peripheral administration of BN. An evaluation of meal patterns showed that GRP-R KO mice ate significantly more at each meal than WT mice, although total 24 h food consumption was equivalent. A long-term analysis of body weight revealed a significant elevation in GRP-R KO mice compared with WT littermates beginning at 45 weeks of age. These data suggest that the GRP-R mediates the feeding effects of BN-like peptides and participates in the termination of meals in mice.

Original languageEnglish (US)
Pages (from-to)273-281
Number of pages9
JournalJournal of Endocrinology
Volume174
Issue number2
DOIs
StatePublished - Aug 2002

Fingerprint

Bombesin Receptors
Gastrin-Releasing Peptide
Bombesin
Body Weight
Meals
Peptides
Solitary Nucleus
Glucose
Paraventricular Hypothalamic Nucleus
Cholecystokinin
Feeding Behavior

ASJC Scopus subject areas

  • Endocrinology

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Disruptions in feeding and body weight control in gastrin-releasing peptide receptor deficient mice. / Ladenheim, Ellen Elizabeth; Hampton, L. L.; Whitney, A. C.; White, W. O.; Battey, J. F.; Moran, Timothy H.

In: Journal of Endocrinology, Vol. 174, No. 2, 08.2002, p. 273-281.

Research output: Contribution to journalArticle

Ladenheim, Ellen Elizabeth ; Hampton, L. L. ; Whitney, A. C. ; White, W. O. ; Battey, J. F. ; Moran, Timothy H. / Disruptions in feeding and body weight control in gastrin-releasing peptide receptor deficient mice. In: Journal of Endocrinology. 2002 ; Vol. 174, No. 2. pp. 273-281.
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