Disruption of the Pfg27 locus by homologous recombination leads to loss of the sexual phenotype in P. falciparum

Cheryl Ann Lobo, Hisashi Fujioka, Masamichi Aikawa, Nirbhay Kumar

Research output: Contribution to journalArticlepeer-review

Abstract

Transmission of malaria depends upon the differentiation and development of the sexual stages of the parasite. In Plasmodium falciparum, it is a complex, multistage process, involving the expression of a large number of sexual stage-specific proteins. Pfg27 is one such protein, abundantly expressed at the onset of gametocytogenesis. We report successful disruption of the Pfg27 locus using homologous recombination and show that it is essential for the maintenance of the sexual phenotype. Transfectants lacking Pfg27 abort early in sexual development, resulting in vacuolated, highly disarranged, and disintegrating parasites. This suggests a critical role for Pfg27 in the sexual development of the parasite.

Original languageEnglish (US)
Pages (from-to)793-798
Number of pages6
JournalMolecular cell
Volume3
Issue number6
DOIs
StatePublished - Jun 1999

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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