Disruption of Mekk2 in mice reveals an unexpected role for MEKK2 in modulating T-cell receptor signal transduction

Zijian Guo, Gavin Clydesdale, Jinke Cheng, Kihwan Kim, Lin Gan, David J. McConkey, Stephen E. Ullrich, Yuan Zhuang, Bing Su

Research output: Contribution to journalArticle


MEKK2 is a member of the mitogen-activated protein kinase (MAPK) kinase kinase gene family involved in regulating multiple MAPK signaling pathways. To elucidate the in vivo function of MEKK2, we generated mice carrying a targeted mutation in the Mekk2 locus. Mekk2-/- mice are viable and fertile. Major subsets of thymic and spleen T cells in Mekk2-deficient mice were indistinguishable from those in wild-type mice. B-cell development appeared to proceed similarly in the bone marrow of Mekk2-deficient and wild-type mice. However, Mekk2-/- T-cell proliferation was augmented in response to anti-CD3 monoclonal antibody (MAb) stimulation, and these T cells produced more interleukin 2 and gamma interferon than did the wild-type T cells, suggesting that MEKK2 may be involved in controlling the strength of T-cell receptor (TCR) signaling. Consistently, Mekk2-/- thymocytes were more susceptible than wild-type thymocytes to anti-CD3 MAb-induced cell death. Furthermore, TCR-mediated c-Jun N-terminal kinase activation was not blocked but moderately enhanced in Mekk2-/- T cells. Neither extracellular signal-regulated kinase nor p38 MAPK activation was affected in Mekk2-/- T cells. In conclusion, we found that MEKK2 may be required for controlling the strength of TCR/CD3 signaling.

Original languageEnglish (US)
Pages (from-to)5761-5768
Number of pages8
JournalMolecular and cellular biology
Issue number16
StatePublished - Aug 7 2002
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Disruption of Mekk2 in mice reveals an unexpected role for MEKK2 in modulating T-cell receptor signal transduction'. Together they form a unique fingerprint.

  • Cite this