Disorganization of cultured vascular endothelial cell monolayers by fibrinogen fragment D

Chi V. Dang, William R. Bell, Donald Kaiser, Albert Wong

Research output: Contribution to journalArticle

Abstract

Fibrinogen fragment D, which is heterogeneous, has several important biological functions. Human fibrinogen fragments D94 (molecular weight, 94,000), D78 (78,000), and E (52,000) were purified. Fragments D78 and D94 but not purified fibrinogen or fragment E specifically caused disorganization of bovine aortic endothelial cells cultured as monolayers. Within 2 hours of exposure to pathophysiological concentrations of fragment D, the confluent endothelial cells retracted from each other and projected pseudopodia. These disturbed cells subsequently became rounded and detached from the substrate. The actin present in stress fibers in stationary monolayer cells was diffusely redistributed in cells with fragment D-induced alterations in morphology. This effect was not observed in monolayers of kidney epithelial cells. The results demonstrate a specific effect of fibrinogen fragment D on the disorganization of cultured vascular endothelial cell monolayers and suggest that fragment D plays a role in the pathogenesis of syndromes with vascular endothelial damage.

Original languageEnglish (US)
Pages (from-to)1487-1490
Number of pages4
JournalScience
Volume227
Issue number4693
StatePublished - 1985

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Endothelial Cells
Stress Fibers
Pseudopodia
Fibrinogen
Blood Vessels
Actins
Molecular Weight
Epithelial Cells
Kidney
fibrinogen D fragment
fibrinogen fragment E

ASJC Scopus subject areas

  • General

Cite this

Dang, C. V., Bell, W. R., Kaiser, D., & Wong, A. (1985). Disorganization of cultured vascular endothelial cell monolayers by fibrinogen fragment D. Science, 227(4693), 1487-1490.

Disorganization of cultured vascular endothelial cell monolayers by fibrinogen fragment D. / Dang, Chi V.; Bell, William R.; Kaiser, Donald; Wong, Albert.

In: Science, Vol. 227, No. 4693, 1985, p. 1487-1490.

Research output: Contribution to journalArticle

Dang, CV, Bell, WR, Kaiser, D & Wong, A 1985, 'Disorganization of cultured vascular endothelial cell monolayers by fibrinogen fragment D', Science, vol. 227, no. 4693, pp. 1487-1490.
Dang CV, Bell WR, Kaiser D, Wong A. Disorganization of cultured vascular endothelial cell monolayers by fibrinogen fragment D. Science. 1985;227(4693):1487-1490.
Dang, Chi V. ; Bell, William R. ; Kaiser, Donald ; Wong, Albert. / Disorganization of cultured vascular endothelial cell monolayers by fibrinogen fragment D. In: Science. 1985 ; Vol. 227, No. 4693. pp. 1487-1490.
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