TY - JOUR
T1 - Disk diffusion bioassays for the detection of antibiotic activity in body fluids
T2 - Applications for the pneumonia etiology research for child health project
AU - Driscoll, Amanda J.
AU - Bhat, Niranjan
AU - Karron, Ruth A.
AU - O'Brien, Katherine L.
AU - Murdoch, David R.
N1 - Funding Information:
Supplement sponsorship. This article was published as part of a supplement entitled ‘‘Pneumonia Etiology Research for Child Health,’’ sponsored by a grant from The Bill & Melinda Gates Foundation to the PERCH Project of Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland. Potential conflicts of interest. All authors: No reported conflicts.
Funding Information:
Financial support. This work was supported by grant 48968 from The Bill & Melinda Gates Foundation to the International Vaccine Access Center, Department of International Health, Johns Hopkins Bloomberg School of Public Health.
PY - 2012/4/1
Y1 - 2012/4/1
N2 - To draw inferences about the putative etiologic agents of severe pneumonia, the Pneumonia Etiology Research for Child Health (PERCH) project must be able to objectively assess antibiotic pretreatment in enrolled participants. This review is focused on the disk diffusion bioassay, a simple laboratory method to assess recent antibiotic treatment. In this method, a sensitive indicator organism is used to detect antimicrobial activity in body fluid specimens that have been inoculated on a filter paper disk and placed on agar growth medium. We reviewed and present several variations on the disk diffusion method as applied to serum or urine, including specimen handling, choice of indicator organism and medium, and incubation steps. Although there are limitations to the disk diffusion method, its low cost, ease of use, and ability to broadly detect antibiotic pretreatment make it an appealing method for epidemiologic studies such as PERCH.
AB - To draw inferences about the putative etiologic agents of severe pneumonia, the Pneumonia Etiology Research for Child Health (PERCH) project must be able to objectively assess antibiotic pretreatment in enrolled participants. This review is focused on the disk diffusion bioassay, a simple laboratory method to assess recent antibiotic treatment. In this method, a sensitive indicator organism is used to detect antimicrobial activity in body fluid specimens that have been inoculated on a filter paper disk and placed on agar growth medium. We reviewed and present several variations on the disk diffusion method as applied to serum or urine, including specimen handling, choice of indicator organism and medium, and incubation steps. Although there are limitations to the disk diffusion method, its low cost, ease of use, and ability to broadly detect antibiotic pretreatment make it an appealing method for epidemiologic studies such as PERCH.
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U2 - 10.1093/cid/cir1061
DO - 10.1093/cid/cir1061
M3 - Article
C2 - 22403231
AN - SCOPUS:84858138260
SN - 1058-4838
VL - 54
SP - S159-S164
JO - Clinical Infectious Diseases
JF - Clinical Infectious Diseases
IS - SUPPL. 2
ER -