Disease activity and cytokine production in mitogen-stimulated peripheral blood mononuclear cells from patients with rheumatoid arthritis

Sukhbir S. Uppal, Raj Raghupathy, Sawsan J. Hayat, Joseph C. Longenecker, Mini Abraham, Parvez Rawoot

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To test whether there are differences in the levels and ratios of 6 pro- and 3 anti-inflammatory cytokines produced by mitogen-stimulated peripheral blood mononuclear cells (PBMCs) in rheumatoid arthritis (RA) subjects compared to controls. Subjects and Methods: 79 participants (42 seropositive RA patients and 37 healthy controls) were enrolled in this study. The production levels in mitogen-stimulated PBMCs of the 6 proinflammatory cytokines (IFN-γ, TNF-α, TNF-β, IL-8, IL-17, IL-18) and 3 anti-inflammatory cytokines (IL-4, IL-10, IL-13) were assayed by ELISA using kits obtained from Immunotech SA. The ratios of pro- to anti-inflammatory cytokines were calculated for all participants. Results: There were significantly elevated levels of IL-8 and IL-10, and reduced levels of IFN-γ, IL-4, and IL-17 in mitogen-stimulated PBMC culture supernatants of RA subjects compared to controls. Of the 18 pro-/anti-inflammatory cytokine ratios, 3 ratios (TNF-α/IL13, IL-8/IL-4 and IL-8/IL-13) were significantly higher in RA patients compared to controls; and 6 were higher in controls (IFN-γ/IL-4; IFN-γ/IL-10; IFN-γ/IL-13; TNF-β/IL10; IL-17/IL-10; IL-18/IL-10). Conclusions:Activated PBMCs of RA patients, regardless of disease activity, showed higher-level production of IL-8 and IL-10 compared to controls; lower-level production of IFN-γ, IL-4, and IL-17; and elevated ratios of TNF-α/IL-13, IL-8/IL-4 and IL-8/IL-13.

Original languageEnglish (US)
Pages (from-to)33-39
Number of pages7
JournalMedical Principles and Practice
Volume19
Issue number1
DOIs
StatePublished - Jan 2009
Externally publishedYes

Keywords

  • Arthritis
  • Cytokines
  • Interferon-γ
  • Peripheral blood mononuclear cells
  • Rheumatoid arthritis

ASJC Scopus subject areas

  • Medicine(all)

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