@article{4191b0bcd2b543748562313bb8606a5e,
title = "Discovery of Ubonodin, an Antimicrobial Lasso Peptide Active against Members of the Burkholderia cepacia Complex",
abstract = "We report the heterologous expression, structure, and antimicrobial activity of a lasso peptide, ubonodin, encoded in the genome of Burkholderia ubonensis. The topology of ubonodin is unprecedented amongst lasso peptides, with 18 of its 28 amino acids found in the mechanically bonded loop segment. Ubonodin inhibits RNA polymerase in vitro and has potent antimicrobial activity against several pathogenic members of the Burkholderia genus, most notably B. cepacia and B. multivorans, causative agents of lung infections in cystic fibrosis patients.",
keywords = "RiPP, antibiotics, lasso peptides, natural products, peptides",
author = "Cheung-Lee, {Wai Ling} and Parry, {Madison E.} and Chuhan Zong and Cartagena, {Alexis Jaramillo} and Darst, {Seth A.} and Connell, {Nancy D.} and Riccardo Russo and Link, {A. James}",
note = "Funding Information: We thank Apichai Tuanyok for testing ubonodin against Bp82. We also thank Herbert Schweizer, Mark Brynildsen, and Mohammad Seyedsayamdost for sharing bacterial strains for ubonodin susceptibility. Finally, we thank Istv{\'a}n Pelczer (Princeton University NMR Facility) for help with acquiring NMR spectra. This work was supported by US National Institutes of Health (NIH) grants GM107036 to A.J.L. and GM118130 to S.A.D. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. This work was also supported in part by Princeton University SEAS Innovation Funds (Focused Research Team for Precision Antibiotics). W.L.C.‐L. was supported in part by a Dodds Fellowship from Princeton University. A.J.C. was supported by a Robert D. Watkins Graduate Research Fellowship from the American Society of Microbiology. B. pseudomallei Publisher Copyright: {\textcopyright} 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2020",
month = may,
day = "4",
doi = "10.1002/cbic.201900707",
language = "English (US)",
volume = "21",
pages = "1335--1340",
journal = "ChemBioChem",
issn = "1439-4227",
publisher = "Wiley-VCH Verlag",
number = "9",
}