Discovery of Ubonodin, an Antimicrobial Lasso Peptide Active against Members of the Burkholderia cepacia Complex

Wai Ling Cheung-Lee, Madison E. Parry, Chuhan Zong, Alexis Jaramillo Cartagena, Seth A. Darst, Nancy D. Connell, Riccardo Russo, A. James Link

Research output: Contribution to journalArticle

Abstract

We report the heterologous expression, structure, and antimicrobial activity of a lasso peptide, ubonodin, encoded in the genome of Burkholderia ubonensis. The topology of ubonodin is unprecedented amongst lasso peptides, with 18 of its 28 amino acids found in the mechanically bonded loop segment. Ubonodin inhibits RNA polymerase in vitro and has potent antimicrobial activity against several pathogenic members of the Burkholderia genus, most notably B. cepacia and B. multivorans, causative agents of lung infections in cystic fibrosis patients.

Original languageEnglish (US)
Pages (from-to)1335-1340
Number of pages6
JournalChemBioChem
Volume21
Issue number9
DOIs
StatePublished - May 4 2020

Keywords

  • RiPP
  • antibiotics
  • lasso peptides
  • natural products
  • peptides

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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  • Cite this

    Cheung-Lee, W. L., Parry, M. E., Zong, C., Cartagena, A. J., Darst, S. A., Connell, N. D., Russo, R., & Link, A. J. (2020). Discovery of Ubonodin, an Antimicrobial Lasso Peptide Active against Members of the Burkholderia cepacia Complex. ChemBioChem, 21(9), 1335-1340. https://doi.org/10.1002/cbic.201900707