Discovery of disease-induced post-translational modifications in cardiac contractile proteins

Chao Yuan, Rajashree Ravi, Anne M. Murphy

Research output: Contribution to journalReview article

Abstract

Post-translational modifications to myofilament proteins are essential for the regulation of cardiac function in both normal and disease states. Recent developments in the field of proteomics have produced a variety of useful tools to study protein modifications. Current applications of proteomic technologies in the study of modifications to myofilament proteins are summarized. The separation, identification and characterization of myofilament modifications using gel electrophoresis and mass spectrometry approaches are discussed. Each method is illustrated and evaluated with selected examples, and several powerful emerging technologies are assessed.

Original languageEnglish (US)
Pages (from-to)234-239
Number of pages6
JournalCurrent Opinion in Molecular Therapeutics
Volume7
Issue number3
StatePublished - Jun 1 2005

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Keywords

  • 2-Dimensional gel electrophoresis
  • Heart disease
  • Mass spectrometry
  • Myofilament proteins
  • Post-translational modifications
  • Proteomics

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Pharmacology
  • Drug Discovery
  • Genetics(clinical)

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