Abstract
Post-translational modifications to myofilament proteins are essential for the regulation of cardiac function in both normal and disease states. Recent developments in the field of proteomics have produced a variety of useful tools to study protein modifications. Current applications of proteomic technologies in the study of modifications to myofilament proteins are summarized. The separation, identification and characterization of myofilament modifications using gel electrophoresis and mass spectrometry approaches are discussed. Each method is illustrated and evaluated with selected examples, and several powerful emerging technologies are assessed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 234-239 |
| Number of pages | 6 |
| Journal | Current Opinion in Molecular Therapeutics |
| Volume | 7 |
| Issue number | 3 |
| State | Published - Jun 2005 |
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Keywords
- 2-Dimensional gel electrophoresis
- Heart disease
- Mass spectrometry
- Myofilament proteins
- Post-translational modifications
- Proteomics
ASJC Scopus subject areas
- Genetics
- Genetics(clinical)
- Pharmacology, Toxicology and Pharmaceutics(all)
Cite this
Discovery of disease-induced post-translational modifications in cardiac contractile proteins. / Yuan, Chao; Ravi, Rajashree; Murphy, Anne M.
In: Current Opinion in Molecular Therapeutics, Vol. 7, No. 3, 06.2005, p. 234-239.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Discovery of disease-induced post-translational modifications in cardiac contractile proteins
AU - Yuan, Chao
AU - Ravi, Rajashree
AU - Murphy, Anne M
PY - 2005/6
Y1 - 2005/6
N2 - Post-translational modifications to myofilament proteins are essential for the regulation of cardiac function in both normal and disease states. Recent developments in the field of proteomics have produced a variety of useful tools to study protein modifications. Current applications of proteomic technologies in the study of modifications to myofilament proteins are summarized. The separation, identification and characterization of myofilament modifications using gel electrophoresis and mass spectrometry approaches are discussed. Each method is illustrated and evaluated with selected examples, and several powerful emerging technologies are assessed.
AB - Post-translational modifications to myofilament proteins are essential for the regulation of cardiac function in both normal and disease states. Recent developments in the field of proteomics have produced a variety of useful tools to study protein modifications. Current applications of proteomic technologies in the study of modifications to myofilament proteins are summarized. The separation, identification and characterization of myofilament modifications using gel electrophoresis and mass spectrometry approaches are discussed. Each method is illustrated and evaluated with selected examples, and several powerful emerging technologies are assessed.
KW - 2-Dimensional gel electrophoresis
KW - Heart disease
KW - Mass spectrometry
KW - Myofilament proteins
KW - Post-translational modifications
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=20344363386&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=20344363386&partnerID=8YFLogxK
M3 - Article
VL - 7
SP - 234
EP - 239
JO - Current Opinion in Molecular Therapeutics
JF - Current Opinion in Molecular Therapeutics
SN - 1464-8431
IS - 3
ER -