Discovery of disease-induced post-translational modifications in cardiac contractile proteins

Chao Yuan; Rajashree Ravi; Anne M. Murphy

Discovery of disease-induced post-translational modifications in cardiac contractile proteins

Chao Yuan, Rajashree Ravi, Anne M. Murphy

School of Medicine

Research output: Contribution to journal › Review article › peer-review

Abstract

Post-translational modifications to myofilament proteins are essential for the regulation of cardiac function in both normal and disease states. Recent developments in the field of proteomics have produced a variety of useful tools to study protein modifications. Current applications of proteomic technologies in the study of modifications to myofilament proteins are summarized. The separation, identification and characterization of myofilament modifications using gel electrophoresis and mass spectrometry approaches are discussed. Each method is illustrated and evaluated with selected examples, and several powerful emerging technologies are assessed.

Original language	English (US)
Pages (from-to)	234-239
Number of pages	6
Journal	Current Opinion in Molecular Therapeutics
Volume	7
Issue number	3
State	Published - Jun 1 2005

Keywords

2-Dimensional gel electrophoresis
Heart disease
Mass spectrometry
Myofilament proteins
Post-translational modifications
Proteomics

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Genetics
Pharmacology
Drug Discovery
Genetics(clinical)

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AB - Post-translational modifications to myofilament proteins are essential for the regulation of cardiac function in both normal and disease states. Recent developments in the field of proteomics have produced a variety of useful tools to study protein modifications. Current applications of proteomic technologies in the study of modifications to myofilament proteins are summarized. The separation, identification and characterization of myofilament modifications using gel electrophoresis and mass spectrometry approaches are discussed. Each method is illustrated and evaluated with selected examples, and several powerful emerging technologies are assessed.

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