Discovering altered genomic expression patterns in heart: Transcriptome determination by serial analysis of gene expression

S. V. Anisimov, Edward Lakatta, K. R. Boheler

Research output: Contribution to journalReview article

Abstract

The development of cardiovascular diseases such as heart failure involve functional changes that are beneficial short-term, but may be fatal long-term. Current therapeutic approaches are tailored to limit progression of a disease and to maintain quality of life. At a molecular level, these disease processes involve quantitative and qualitative changes in gene expression. Although some changes in mRNA abundance may not have direct protein correlates, analysis of all the mRNAs present in a cell population (the cells transcriptome) has become a focal point of genomic research. The aim is to provide information about the dynamics of total genome expression in response to environmental changes and point to candidate genes responsible for the cascade of events that result in a disease state. One way of performing these analyses utilizes the technique of Serial Analysis of Gene Expression (SAGE). This method evaluates thousands of expressed transcripts both quantitatively and qualitatively in a single assay. In the first of two reviews on transcriptome analysis, we describe the current state of genomic research for determination of the transcriptome by Serial Analysis of Gene Expression, present the first limited SAGE analysis of rodent heart gene expression, and discuss how results generated with this approach can be applied to the study and treatment of cardiovascular diseases. Published by Elsevier Science Ireland ltd on behalf of European Society of Cardiology.

Original languageEnglish (US)
Pages (from-to)271-281
Number of pages11
JournalEuropean Journal of Heart Failure
Volume3
Issue number3
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Transcriptome
Gene Expression
Gene Expression Profiling
Cardiovascular Diseases
Messenger RNA
Ireland
Research
Disease Progression
Rodentia
Heart Failure
Quality of Life
Genome
Population
Genes
Proteins
Therapeutics

Keywords

  • Heart Failure
  • Human
  • Rodent
  • SAGE
  • Transcriptome

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Discovering altered genomic expression patterns in heart : Transcriptome determination by serial analysis of gene expression. / Anisimov, S. V.; Lakatta, Edward; Boheler, K. R.

In: European Journal of Heart Failure, Vol. 3, No. 3, 2001, p. 271-281.

Research output: Contribution to journalReview article

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