Discordant effects of aging on prolactin and luteinizing hormone-β messenger ribonucleic acid levels in the female rat

David A. Stewart, Marc R. Blackman, Mary Ann Kowatch, David B. Danner, George S. Roth

Research output: Contribution to journalArticle

Abstract

To examine the molecular genetic basis for the age-related increase in PRL secretion and decrease in LH production in the rat, we measured steady state levels of PRL and LHβ mRNA in pituitary homogenates and cell lysates from monolayer adenohypophyseal cultures. These mRNA levels were compared with the corresponding levels of immunoreactive PRL and LH in sera and culture media. Paired groups (n = 4-10/ group) of intact and 4-week ovariectomized mature (6-7 months old) and old (23-25 months old) female Wistar rats were studied. Serum PRL levels were 550% higher in intact old vs. mature rats (P <0.001), whereas the corresponding pituitary homogenate levels of PRL mRNA were similar (P > 0.4). Medium PRL concentrations were 230% greater (P <0.006) whereas cell lysate concentrations of PRL mRNA were unaltered (P > 0.2) in monolayer cultures from intact old vs. mature rats. Serum PRL levels were 650% higher (P <0.003) and pituitary homogenate PRL mRNA levels were slightly increased (P <0.04) in ovariectomized old vs. mature rats. Neither serum LH values (P > 0.07) nor pituitary homogenate LHβ mRNA levels (P > 0.1) differed in intact old and mature rats, whereas the corresponding medium concentrations of LH were reduced (P <0.001). Ovariectomized old vs. mature rats exhibited reductions in serum (P <0.02) and medium (P <0.001) LH concentrations, as well as in pituitary homogenate (P <0.002) and cell lysate (P <0.006) LHβ mRNA levels. Thus, these data revealed coordinate decreases with age in LHβ mRNA and LH secretion, particularly in ovariectomized rats, suggesting an age-related alteration at or before LHβ gene transcription. These findings parallel observations on other genes whose products change with age. In contrast, the observation that the increased secretion of PRL in old rats is accompanied by little or no increase in PRL mRNA is novel and suggests that age-related alterations in PRL gene expression proceed through a posttranscriptional mechanism.

Original languageEnglish (US)
Pages (from-to)773-778
Number of pages6
JournalEndocrinology
Volume126
Issue number2
StatePublished - Feb 1990

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Luteinizing Hormone
Prolactin
RNA
Messenger RNA
Serum
Genes
Culture Media
Wistar Rats
Molecular Biology
Gene Expression

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Discordant effects of aging on prolactin and luteinizing hormone-β messenger ribonucleic acid levels in the female rat. / Stewart, David A.; Blackman, Marc R.; Kowatch, Mary Ann; Danner, David B.; Roth, George S.

In: Endocrinology, Vol. 126, No. 2, 02.1990, p. 773-778.

Research output: Contribution to journalArticle

Stewart, David A. ; Blackman, Marc R. ; Kowatch, Mary Ann ; Danner, David B. ; Roth, George S. / Discordant effects of aging on prolactin and luteinizing hormone-β messenger ribonucleic acid levels in the female rat. In: Endocrinology. 1990 ; Vol. 126, No. 2. pp. 773-778.
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abstract = "To examine the molecular genetic basis for the age-related increase in PRL secretion and decrease in LH production in the rat, we measured steady state levels of PRL and LHβ mRNA in pituitary homogenates and cell lysates from monolayer adenohypophyseal cultures. These mRNA levels were compared with the corresponding levels of immunoreactive PRL and LH in sera and culture media. Paired groups (n = 4-10/ group) of intact and 4-week ovariectomized mature (6-7 months old) and old (23-25 months old) female Wistar rats were studied. Serum PRL levels were 550{\%} higher in intact old vs. mature rats (P <0.001), whereas the corresponding pituitary homogenate levels of PRL mRNA were similar (P > 0.4). Medium PRL concentrations were 230{\%} greater (P <0.006) whereas cell lysate concentrations of PRL mRNA were unaltered (P > 0.2) in monolayer cultures from intact old vs. mature rats. Serum PRL levels were 650{\%} higher (P <0.003) and pituitary homogenate PRL mRNA levels were slightly increased (P <0.04) in ovariectomized old vs. mature rats. Neither serum LH values (P > 0.07) nor pituitary homogenate LHβ mRNA levels (P > 0.1) differed in intact old and mature rats, whereas the corresponding medium concentrations of LH were reduced (P <0.001). Ovariectomized old vs. mature rats exhibited reductions in serum (P <0.02) and medium (P <0.001) LH concentrations, as well as in pituitary homogenate (P <0.002) and cell lysate (P <0.006) LHβ mRNA levels. Thus, these data revealed coordinate decreases with age in LHβ mRNA and LH secretion, particularly in ovariectomized rats, suggesting an age-related alteration at or before LHβ gene transcription. These findings parallel observations on other genes whose products change with age. In contrast, the observation that the increased secretion of PRL in old rats is accompanied by little or no increase in PRL mRNA is novel and suggests that age-related alterations in PRL gene expression proceed through a posttranscriptional mechanism.",
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