Dirofilaria immitis: Studies on anti-microfilarial immunity in Lewis rats

W. K. Tamashiro, M. S. Ibrahim, D. A. Moraga, A. L. Scott

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of vaccination on rates of microfilarial clearance using Dirofilaria immitis in male Lewis rats was examined. Animals were immunized with whole, dead microfilariae or a PBS extract of microfilariae in Freund's adjuvant. The immunized animals, as well as untreated and adjuvant controls, were challenged intravenously with 4 x 105 viable microfilariae (mf). The duration of microfilaremia was 15.5 days in rats vaccinated with whole mf, 17.7 days in those vaccinated with a PBS extract, 36.3 days for those vaccinated with adjuvant alone, and > 70 days for the untreated group. Analysis of the anti-microfilarial IgG response by ELISA and Western blots demonstrated that immunization induced significant amounts of antibody against high molecular weight peptides, particularly a peptide located at 105 kDa. Antibody levels in both groups of immunized animals continued to rise following challenge, reaching peak levels of 78-80 μg/ml on the day of microfilarial clearance. Decreasing microfilaremia following challenge was associated with an enhanced recognition of low molecular weight peptides.

Original languageEnglish (US)
Pages (from-to)368-376
Number of pages9
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume40
Issue number4
DOIs
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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