Dirofilaria immitis: Identification and characterization of circulating parasite antigens

John P. Ehrenberg, Wesley K. Tamashiro, Alan L. Scott

Research output: Contribution to journalArticlepeer-review

Abstract

A method for the identification of circulating parasite antigens in filarial nematode infections was developed using canine infections with Dirofilaria immitis as a model. Filarial antigens ranging in molecular weight from 211 to 13 kDa were extracted from the sera of microfilaremic dogs by a solid phase immunobinding procedure and identified by immunostaining of Western blots. A major antigen of 104 kDa was selected for further characterization. The 104 kDa circulating antigen showed antigenic and biochemical identity with 104 kDa peptides found in extracts of adult male and microfilarial stages of the parasite. The 104 kDa peptide was antigenically stable under a variety of storage conditions. Its potential as a diagnostic target is discussed.

Original languageEnglish (US)
Pages (from-to)205-214
Number of pages10
JournalExperimental Parasitology
Volume63
Issue number2
DOIs
StatePublished - Apr 1987

Keywords

  • Antigen detection assay
  • Circulating antigen
  • Cross reacting antigens
  • Dirofilaria immitis
  • Dog
  • Filarioidea
  • Immune complexed antigen
  • Nematode, parasitic

ASJC Scopus subject areas

  • Parasitology
  • Immunology
  • Infectious Diseases

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