Dirofilaria immitis: Biochemical and immunological characterization of the surface antigens from adult parasites

Alan L. Scott, Chamberlain Diala, David A. Moraga, M. S. Ibrahim, Lydia Redding, Wesley K. Tamashiro

Research output: Contribution to journalArticlepeer-review

Abstract

The molecules associated with the surface of adult Dirofilaria immitis were identified and characterized employing IODO-GEN-mediated surface labeling methods. D. immitis female and male parasites were found to have a limited number of surface-associated proteins (17.5,16, and 14.5 kDa) and glycoproteins (49 and 20 kDa) which were readily extracted from parasite homogenates in the absence of detergent. The major surface labeled proteins and glycoproteins were antigenic in rabbits, but appeared to elicit only a weak humoral response in dogs with patent dirofilariasis. In addition, a 10- to 6-kDa surface-associated glycolipid was identified which may form a coat on the outside of the parasite and play a role in immune evasion. In immunoprecipitation experiments, the glycolipid was not recognized by the antibodies from rabbits exposed to the glycolipid or by antibodies in the sera of patently infected animals. The glycolipid and the 14.5-kDa surface protein were selectively released by the adult parasite during in vitro culture.

Original languageEnglish (US)
Pages (from-to)307-323
Number of pages17
JournalExperimental Parasitology
Volume67
Issue number2
DOIs
StatePublished - Dec 1988

Keywords

  • Collagen
  • Dirofilaria immitis
  • Excretory/secretory product
  • Filarioidea
  • Glycolipid
  • Immune evasion
  • Nematode, parasitic
  • Surface antigens, glycoproteins

ASJC Scopus subject areas

  • Parasitology
  • Immunology
  • Infectious Diseases

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