Directed proteomic analysis of the human nucleolus

Jens S. Andersen, Carol E. Lyon, Archa H. Fox, Anthony Kar Lun Leung, Yun Wah Lam, Hanno Steen, Matthias Mann, Angus I. Lamond

Research output: Contribution to journalArticle

Abstract

Background: The nucleolus is a subnuclear organelle containing the ribosomal RNA gene clusters and ribosome biogenesis factors. Recent studies suggest it may also have roles in RNA transport, RNA modification, and cell cycle regulation. Despite over 150 years of research into nucleoli, many aspects of their structure and function remain uncharacterized. Results: We report a proteomic analysis of human nucleoli. Using a combination of mass spectrometry (MS) and sequence database searches, including online analysis of the draft human genome sequence, 271 proteins were identified. Over 30% of the nucleolar proteins were encoded by novel or uncharacterized genes, while the known proteins included several unexpected factors with no previously known nucleolar functions. MS analysis of nucleoli isolated from HeLa cells in which transcription had been inhibited showed that a subset of proteins was enriched. These data highlight the dynamic nature of the nucleolar proteome and show that proteins can either associate with nucleoli transiently or accumulate only under specific metabolic conditions. Conclusions: This extensive proteomic analysis shows that nucleoli have a surprisingly large protein complexity. The many novel factors and separate classes of proteins identified support the view that the nucleolus may perform additional functions beyond its known role in ribosome subunit biogenesis. The data also show that the protein composition of nucleoli is not static and can alter significantly in response to the metabolic state of the cell.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalCurrent Biology
Volume12
Issue number1
DOIs
StatePublished - Jan 8 2002
Externally publishedYes

Fingerprint

cell nucleolus
Proteomics
proteomics
Proteins
Genes
proteins
Mass spectrometry
ribosomes
Mass Spectrometry
RNA Transport
RNA
Ribosome Subunits
mass spectrometry
Ribosomal RNA
Human Genome
Proteome
Transcription
Multigene Family
Nuclear Proteins
Ribosomes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Andersen, J. S., Lyon, C. E., Fox, A. H., Leung, A. K. L., Lam, Y. W., Steen, H., ... Lamond, A. I. (2002). Directed proteomic analysis of the human nucleolus. Current Biology, 12(1), 1-11. https://doi.org/10.1016/S0960-9822(01)00650-9

Directed proteomic analysis of the human nucleolus. / Andersen, Jens S.; Lyon, Carol E.; Fox, Archa H.; Leung, Anthony Kar Lun; Lam, Yun Wah; Steen, Hanno; Mann, Matthias; Lamond, Angus I.

In: Current Biology, Vol. 12, No. 1, 08.01.2002, p. 1-11.

Research output: Contribution to journalArticle

Andersen, JS, Lyon, CE, Fox, AH, Leung, AKL, Lam, YW, Steen, H, Mann, M & Lamond, AI 2002, 'Directed proteomic analysis of the human nucleolus', Current Biology, vol. 12, no. 1, pp. 1-11. https://doi.org/10.1016/S0960-9822(01)00650-9
Andersen, Jens S. ; Lyon, Carol E. ; Fox, Archa H. ; Leung, Anthony Kar Lun ; Lam, Yun Wah ; Steen, Hanno ; Mann, Matthias ; Lamond, Angus I. / Directed proteomic analysis of the human nucleolus. In: Current Biology. 2002 ; Vol. 12, No. 1. pp. 1-11.
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