Direct translation of climbing fiber burst-mediated sensory coding into post-synaptic purkinje cell dendritic calcium

Seung Eon Roh, Seung Ha Kim, Changhyeon Ryu, Chang Eop Kim, Yong Gyu Kim, Paul F. Worley, Sun Kwang Kim, Sang Jeong Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Climbing fibers (CFs) generate complex spikes (CS) and Ca2+ transients in cerebellar Purkinje cells (PCs), serving as instructive signals. The so-called “all-or-none” character of CSs has been questioned since the CF burst was described. Although recent studies have indicated a sensory-driven enhancement of PC Ca2+ signals, how CF responds to sensory events and contributes to PC dendritic Ca2+ and CS remains unexplored. Here, single or simultaneous Ca2+ imaging of CFs and PCs in awake mice revealed the presynaptic CF Ca2+ amplitude encoded the sensory input’s strength and directly influenced post-synaptic PC dendritic Ca2+ amplitude. The sensory-driven variability in CF Ca2+ amplitude depended on the number of spikes in the CF burst. Finally, the spike number of the CF burst determined the PC Ca2+ influx and CS properties. These results reveal the direct translation of sensory information-coding CF inputs into PC Ca2+, suggesting the sophisticated role of CFs as error signals.

Original languageEnglish (US)
Article numbere61593
Pages (from-to)1-27
Number of pages27
JournaleLife
Volume9
DOIs
StatePublished - Sep 2020

Keywords

  • Ca signal
  • Cerebellum
  • Climbing fiber
  • Plasticity
  • Purkinje cell
  • Sensory coding

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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