Direct regulation of GnRH transcription by CRF-like peptides in an immortalized neuronal cell line

D. J. Tellam, M. J. Perone, I. C. Dunn, S. Radovick, J. Brennand, J. E. Rivier, M. G. Castro, D. A. Lovejoy

Research output: Contribution to journalArticlepeer-review

Abstract

The existence of a CRF-dependent inhibition of GnRH transcription was investigated using a neuronal GnRH-expressing cell line (Gn11) stably transfected with mouse (-611 bp) or chicken (-3000 bp) GnRH promoter/luciferase reporter constructs. The presence of the CRF-R1 receptor was established using a specific CRF-R1 antiserum. After 7 h of incubation, urotensin-I and sauvagine increased the mouse GnRH-reporter bioluminescence by 1.3- and 1.2-fold, respectively, compared with control cells. Subsequently, CRF, urotensin-I and sauvagine decreased luciferase reporter activity to about 60% of the control values after 14 h. Similar trends occurred with the chicken GnRH promoter with UI increasing reporter gene activity 2.4-fold over the controls after 14 h incubation. These data provide additional evidence for the direct regulation of GnRH transcription by CRF- like peptides.

Original languageEnglish (US)
Pages (from-to)3135-3140
Number of pages6
JournalNeuroReport
Volume9
Issue number14
StatePublished - Oct 5 1998
Externally publishedYes

Keywords

  • Reproduction
  • Sauvagine
  • Stress
  • Transcription
  • Urotensin-I

ASJC Scopus subject areas

  • Neuroscience(all)

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