Direct radioactive labeling of unpurified PCR products using Klenow fragment

T. K. McDaniel; Y. Huang; J. Yin; S. W. Needleman; S. J. Meltzer

Direct radioactive labeling of unpurified PCR products using Klenow fragment

T. K. McDaniel, Y. Huang, J. Yin, S. W. Needleman, S. J. Meltzer

Research output: Contribution to journal › Article › peer-review

Abstract

We describe a method for rapid radioactive labeling of PCR product. The method, employing the Klenow fragment of DNA polymerase I, consumes little product, requires no product purification and takes under 30 minutes.

Original language	English (US)
Pages (from-to)	164+166
Journal	BioTechniques
Volume	11
Issue number	2
State	Published - Sep 13 1991
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry, Genetics and Molecular Biology(all)

Cite this

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title = "Direct radioactive labeling of unpurified PCR products using Klenow fragment",

abstract = "We describe a method for rapid radioactive labeling of PCR product. The method, employing the Klenow fragment of DNA polymerase I, consumes little product, requires no product purification and takes under 30 minutes.",

author = "McDaniel, {T. K.} and Y. Huang and J. Yin and Needleman, {S. W.} and Meltzer, {S. J.}",

year = "1991",

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AU - McDaniel, T. K.

AU - Huang, Y.

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AU - Needleman, S. W.

AU - Meltzer, S. J.

PY - 1991/9/13

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N2 - We describe a method for rapid radioactive labeling of PCR product. The method, employing the Klenow fragment of DNA polymerase I, consumes little product, requires no product purification and takes under 30 minutes.

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Direct radioactive labeling of unpurified PCR products using Klenow fragment

Abstract

ASJC Scopus subject areas

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