Direct radioactive labeling of unpurified PCR products using Klenow fragment

T. K. McDaniel, Y. Huang, J. Yin, S. W. Needleman, S. J. Meltzer

Research output: Contribution to journal › Article

Abstract

We describe a method for rapid radioactive labeling of PCR product. The method, employing the Klenow fragment of DNA polymerase I, consumes little product, requires no product purification and takes under 30 minutes.

Original languageEnglish (US)
Pages (from-to)164+166
JournalBioTechniques
Volume11
Issue number2
StatePublished - Sep 13 1991
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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    McDaniel, T. K., Huang, Y., Yin, J., Needleman, S. W., & Meltzer, S. J. (1991). Direct radioactive labeling of unpurified PCR products using Klenow fragment. BioTechniques, 11(2), 164+166.