TY - JOUR
T1 - Direct profiling of lipid distribution in brain tissue using MALDI-TOFMS
AU - Jackson, Shelley N.
AU - Wang, Hay Yan J
AU - Woods, Amina S.
PY - 2005/7/15
Y1 - 2005/7/15
N2 - Recent developments in mass spectrometry have permitted direct analysis of biomolecules in tissue. However, most studies have focused on proteins with emphasis on biomarker discovery. In the present work, matrix-assisted laser desorption/ionization mass spectrometry was used for the direct analysis of lipids in rat cerebellum. The lipid bilayer role as a storage depot for small organic molecules such as therapeutic drugs and pollutants such as DDT, as well as the ability to compare lipid profiles in healthy and diseased animal models, are a few of the many reasons why the direct probing of tissue to determine the qualitative and possibly quantitative lipid content could be a very useful tool. Molecular ions corresponding to cholesterol, phosphatidylcholines, sphingomyelins, and phosphatidylethanolamines were recorded in positive ion mode, while ones corresponding to phosphatidylinositols, sulfatides, and gangliosides were recorded in negative ion mode. Overall, representatives from all major categories of brain lipids including cholesterol, 15 phospholipid species (9 phosphatidylcholines, 1 sphingomyelin, 3 phosphatidylethanolamines, 2 phosphatidylinositols), 10 sulfatides (5 hydroxylated species and 5 nonhydroxylated species), and 7 species of gangliosides were detected.
AB - Recent developments in mass spectrometry have permitted direct analysis of biomolecules in tissue. However, most studies have focused on proteins with emphasis on biomarker discovery. In the present work, matrix-assisted laser desorption/ionization mass spectrometry was used for the direct analysis of lipids in rat cerebellum. The lipid bilayer role as a storage depot for small organic molecules such as therapeutic drugs and pollutants such as DDT, as well as the ability to compare lipid profiles in healthy and diseased animal models, are a few of the many reasons why the direct probing of tissue to determine the qualitative and possibly quantitative lipid content could be a very useful tool. Molecular ions corresponding to cholesterol, phosphatidylcholines, sphingomyelins, and phosphatidylethanolamines were recorded in positive ion mode, while ones corresponding to phosphatidylinositols, sulfatides, and gangliosides were recorded in negative ion mode. Overall, representatives from all major categories of brain lipids including cholesterol, 15 phospholipid species (9 phosphatidylcholines, 1 sphingomyelin, 3 phosphatidylethanolamines, 2 phosphatidylinositols), 10 sulfatides (5 hydroxylated species and 5 nonhydroxylated species), and 7 species of gangliosides were detected.
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U2 - 10.1021/ac050276v
DO - 10.1021/ac050276v
M3 - Article
C2 - 16013869
AN - SCOPUS:27544499300
SN - 0003-2700
VL - 77
SP - 4523
EP - 4527
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 14
ER -