Direct ovarian effect of clomiphene citrate in the rabbit

Y. Yoshimura, H. Kitai, R. Santulli, K. Wright, Edward E Wallach

Research output: Contribution to journalArticle

Abstract

The effects of clomiphene citrate (CC) on ovulation and ovum maturation were studied using the isolated perfused rabbit ovary. CC (10-5 M) added to the perfusate with human chorionic gonadotropin (50 IU) did not affect ovulatory efficiency, ovulation time, oocyte maturation, or degeneration of ovulated ova and follicular oocytes. During perfusion without human chorionic gonadotropin, the percentage of follicular oocytes with germinal vesicle breakdown was significantly increased in response to CC (10-5 M or 10-7 M); a greater percentage of follicular oocytes was degenerated. Estradiol (100 ng/ml) added to the perfusate reversed the effect of CC on degeneration of follicular oocytes. Of follicular oocytes from ovaries perfused with CC, 79.3% were degenerated; in contrast, 25% were degenerated in ovaries treated with CC plus estradiol. These data suggest that CC has a direct ovarian effect and that ovum degeneration associated with CC may be related to an antiestrogenic action.

Original languageEnglish (US)
Pages (from-to)471-476
Number of pages6
JournalFertility and Sterility
Volume43
Issue number3
StatePublished - 1985
Externally publishedYes

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Clomiphene
Rabbits
Oocytes
Ovum
Ovary
Chorionic Gonadotropin
Ovulation
Estradiol
Perfusion

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Yoshimura, Y., Kitai, H., Santulli, R., Wright, K., & Wallach, E. E. (1985). Direct ovarian effect of clomiphene citrate in the rabbit. Fertility and Sterility, 43(3), 471-476.

Direct ovarian effect of clomiphene citrate in the rabbit. / Yoshimura, Y.; Kitai, H.; Santulli, R.; Wright, K.; Wallach, Edward E.

In: Fertility and Sterility, Vol. 43, No. 3, 1985, p. 471-476.

Research output: Contribution to journalArticle

Yoshimura, Y, Kitai, H, Santulli, R, Wright, K & Wallach, EE 1985, 'Direct ovarian effect of clomiphene citrate in the rabbit', Fertility and Sterility, vol. 43, no. 3, pp. 471-476.
Yoshimura Y, Kitai H, Santulli R, Wright K, Wallach EE. Direct ovarian effect of clomiphene citrate in the rabbit. Fertility and Sterility. 1985;43(3):471-476.
Yoshimura, Y. ; Kitai, H. ; Santulli, R. ; Wright, K. ; Wallach, Edward E. / Direct ovarian effect of clomiphene citrate in the rabbit. In: Fertility and Sterility. 1985 ; Vol. 43, No. 3. pp. 471-476.
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