Direct Observation of Calcium Oxalate Monohydrate Precipitation at Phospholipid Monolayers with Brewster Angle Microscopy

Isa O. Benítez, Rénal Backov, Saeed R. Khan, Daniel R. Talham

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The precipitation of calcium oxalate monohydrate (COM) at phospholipid monolayers has been observed in-situ by Brewster angle microscopy (BAM). A monolayer of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) compressed to a LC state over a calcium oxalate subphase shows the growth of COM as very bright spots. The identity of COM was confirmed in a transferred film by scanning electron microscopy. BAM can also be used to determine where COM precipitates when the monolayer has two phases at equilibrium. Monolayers of DPPC and 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (DPPG) crystallize COM at phase boundaries. In addition, phase separated binary phospholipid mixtures of DPPC and 1,2-dimiristoyl-sn-glycero-3-phosphocholine (DMPC) have been prepared and monitored by BAM. The crystal growth in this case is confined to domains of DPPC due to its ability to form a liquid condensed phase.

Original languageEnglish (US)
Title of host publicationMaterials Research Society Symposium - Proceedings
EditorsJ.L. Thomas, K.L. Kiick, L.A. Gower
Pages209-215
Number of pages7
Volume774
StatePublished - 2003
Externally publishedYes
EventMATERIALS RESEARCH SOCIETY SYMPOSIUM PROCEEDINGS: Materials Inspired by Biology - San Francisco, CA, United States
Duration: Apr 21 2003Apr 25 2003

Other

OtherMATERIALS RESEARCH SOCIETY SYMPOSIUM PROCEEDINGS: Materials Inspired by Biology
CountryUnited States
CitySan Francisco, CA
Period4/21/034/25/03

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials

Fingerprint Dive into the research topics of 'Direct Observation of Calcium Oxalate Monohydrate Precipitation at Phospholipid Monolayers with Brewster Angle Microscopy'. Together they form a unique fingerprint.

Cite this