Direct modulation of secretory chloride channels by arachidonic and other cis unsaturated fatty acids

T. C. Hwang, S. E. Guggino, W. B. Guggino

Research output: Contribution to journalArticle

Abstract

The effect of fatty acids on Cl- channels and transepithelial Cl- secretion is investigated. Patch-clamp experiments show that arachidonic acid blocks Cl- channels in a dose-dependent manner. Kinetic analysis shows that the mean open time is decreased 10-fold with 25 μM arachidonic acid. There is a linear relationship between the reciprocal of mean open time and blocker concentration within the range of 1 to 25 μM. The reciprocal of mean blocked time does not change with arachidonic acid concentration. Other cis unsaturated fatty acids, including oleic, linoleic, and ricinoleic acids, demonstrate similar blocks. Trans unsaturated acids such as elaidic acid and saturated fatty acids, including stearic, palmitic, and myristic acids, do not inhibit the channel at 20 μM. Ricinoleic acid decreases short circuit current in T84 cells, a colonic carcinoma cell line that secretes Cl-. Our results suggest that the direct effect of arachidonic and other fatty acids on Cl- secretion is to block Cl- channel current.

Original languageEnglish (US)
Pages (from-to)5706-5709
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number15
DOIs
StatePublished - Aug 23 1990

Keywords

  • T cells
  • asthma
  • colonic epithelia
  • cystic fibrosis
  • tracheal epithelia

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Direct modulation of secretory chloride channels by arachidonic and other cis unsaturated fatty acids'. Together they form a unique fingerprint.

  • Cite this