Direct measurement of the initial proton extrusion to oxygen uptake ratio accompanying succinate oxidation by rat liver mitochondria.

O. H. Setty, R. I. Shrager, B. Bunow, B. Reynafarje, A. L. Lehninger, R. W. Hendler

Research output: Contribution to journalArticle

Abstract

The problem of obtaining very early ratios for the H+/O stoichiometry accompanying succinate oxidation by rat liver mitochondria was attacked using new techniques for direct measurement rather than extrapolations based on data obtained after mixing and the recovery of the electrode from initial injection of O2. Respiration was quickly initiated in a thoroughly mixed O2-containing suspension of mitochondria under a CO atmosphere by photolysis of the CO-cytochrome c oxidase complex-. Fast responding O2 and pH electrodes were used to collect data every 10 ms. The response time for each electrode was experimentally measured in each experiment and suitable corrections for electrode relaxations were made. With uncorrected data obtained after 0.8 s, the extrapolation back to zero time on the basis of single-exponential curve fitting confirmed values close to 8.0 as previously reported (Costa et al., 1984). The data directly obtained, however, indicate an initial burst in H+/O ratio that peaked to values of approximately 20 to 30 prior to 50 ms and which was no longer evident after 0.3 s. Newer information and considerations that place all extrapolation methods in question are discussed.

Original languageEnglish (US)
Pages (from-to)391-404
Number of pages14
JournalBiophysical Journal
Volume50
Issue number3
StatePublished - Sep 1986
Externally publishedYes

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Liver Mitochondrion
Succinic Acid
Protons
Electrodes
Oxygen
Carbon Monoxide
Photolysis
Electron Transport Complex IV
Atmosphere
Reaction Time
Suspensions
Mitochondria
Respiration
Injections

ASJC Scopus subject areas

  • Biophysics

Cite this

Setty, O. H., Shrager, R. I., Bunow, B., Reynafarje, B., Lehninger, A. L., & Hendler, R. W. (1986). Direct measurement of the initial proton extrusion to oxygen uptake ratio accompanying succinate oxidation by rat liver mitochondria. Biophysical Journal, 50(3), 391-404.

Direct measurement of the initial proton extrusion to oxygen uptake ratio accompanying succinate oxidation by rat liver mitochondria. / Setty, O. H.; Shrager, R. I.; Bunow, B.; Reynafarje, B.; Lehninger, A. L.; Hendler, R. W.

In: Biophysical Journal, Vol. 50, No. 3, 09.1986, p. 391-404.

Research output: Contribution to journalArticle

Setty, OH, Shrager, RI, Bunow, B, Reynafarje, B, Lehninger, AL & Hendler, RW 1986, 'Direct measurement of the initial proton extrusion to oxygen uptake ratio accompanying succinate oxidation by rat liver mitochondria.', Biophysical Journal, vol. 50, no. 3, pp. 391-404.
Setty, O. H. ; Shrager, R. I. ; Bunow, B. ; Reynafarje, B. ; Lehninger, A. L. ; Hendler, R. W. / Direct measurement of the initial proton extrusion to oxygen uptake ratio accompanying succinate oxidation by rat liver mitochondria. In: Biophysical Journal. 1986 ; Vol. 50, No. 3. pp. 391-404.
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