Direct measurement of extracellular electrical signals from mammalian olfactory sensory neurons in planar triode devices

Hwajeong Kim, So Yeun Kim, Sungho Nam, Gabriele V. Ronnett, Hyung Soo Han, Cheil Moon, Youngkyoo Kim

Research output: Contribution to journalArticle

Abstract

An artificial nose was developed to mimic aspects of sensory transduction of the peripheral mammalian olfactory system. We directly cultured and differentiated rat olfactory sensory neurons (OSNs) on indium-tin oxide electrodes of planar triode substrates without a coupling agent. Direct voltage (∼50 μV) and current (∼250 nA) signals were measured simultaneously when OSNs on the planar triode substrates were exposed to odorant mixtures. The response signals were sensitive to the concentration of the odorant mixture, with a typical lifetime, shape, and adaptation profile as seen in responses upon repeated stimulation in vivo. We found that the rising time to the peak current was ∼161 ms, while the signal back to baseline was in 1.8 s, which are in agreement with the natural intracellular electrophysiological responses. These results provide the first evidence that mature OSNs grown in a planar triode device are able to detect direct electrophysiological responses to odorants.

Original languageEnglish (US)
Pages (from-to)2047-2053
Number of pages7
JournalThe Analyst
Volume137
Issue number9
DOIs
StatePublished - May 7 2012

Fingerprint

Olfactory Receptor Neurons
Triodes
Neurons
Equipment and Supplies
Electronic Nose
substrate
indium
Coupling agents
Substrates
Tin oxides
tin
Indium
Rats
Electrodes
electrode
oxide
Electric potential
Odorants

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Electrochemistry
  • Biochemistry
  • Environmental Chemistry

Cite this

Direct measurement of extracellular electrical signals from mammalian olfactory sensory neurons in planar triode devices. / Kim, Hwajeong; Kim, So Yeun; Nam, Sungho; Ronnett, Gabriele V.; Han, Hyung Soo; Moon, Cheil; Kim, Youngkyoo.

In: The Analyst, Vol. 137, No. 9, 07.05.2012, p. 2047-2053.

Research output: Contribution to journalArticle

Kim, Hwajeong ; Kim, So Yeun ; Nam, Sungho ; Ronnett, Gabriele V. ; Han, Hyung Soo ; Moon, Cheil ; Kim, Youngkyoo. / Direct measurement of extracellular electrical signals from mammalian olfactory sensory neurons in planar triode devices. In: The Analyst. 2012 ; Vol. 137, No. 9. pp. 2047-2053.
@article{6c665a47d26e48b49cb43b8d1dbf88ee,
title = "Direct measurement of extracellular electrical signals from mammalian olfactory sensory neurons in planar triode devices",
abstract = "An artificial nose was developed to mimic aspects of sensory transduction of the peripheral mammalian olfactory system. We directly cultured and differentiated rat olfactory sensory neurons (OSNs) on indium-tin oxide electrodes of planar triode substrates without a coupling agent. Direct voltage (∼50 μV) and current (∼250 nA) signals were measured simultaneously when OSNs on the planar triode substrates were exposed to odorant mixtures. The response signals were sensitive to the concentration of the odorant mixture, with a typical lifetime, shape, and adaptation profile as seen in responses upon repeated stimulation in vivo. We found that the rising time to the peak current was ∼161 ms, while the signal back to baseline was in 1.8 s, which are in agreement with the natural intracellular electrophysiological responses. These results provide the first evidence that mature OSNs grown in a planar triode device are able to detect direct electrophysiological responses to odorants.",
author = "Hwajeong Kim and Kim, {So Yeun} and Sungho Nam and Ronnett, {Gabriele V.} and Han, {Hyung Soo} and Cheil Moon and Youngkyoo Kim",
year = "2012",
month = "5",
day = "7",
doi = "10.1039/c2an16205a",
language = "English (US)",
volume = "137",
pages = "2047--2053",
journal = "The Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",
number = "9",

}

TY - JOUR

T1 - Direct measurement of extracellular electrical signals from mammalian olfactory sensory neurons in planar triode devices

AU - Kim, Hwajeong

AU - Kim, So Yeun

AU - Nam, Sungho

AU - Ronnett, Gabriele V.

AU - Han, Hyung Soo

AU - Moon, Cheil

AU - Kim, Youngkyoo

PY - 2012/5/7

Y1 - 2012/5/7

N2 - An artificial nose was developed to mimic aspects of sensory transduction of the peripheral mammalian olfactory system. We directly cultured and differentiated rat olfactory sensory neurons (OSNs) on indium-tin oxide electrodes of planar triode substrates without a coupling agent. Direct voltage (∼50 μV) and current (∼250 nA) signals were measured simultaneously when OSNs on the planar triode substrates were exposed to odorant mixtures. The response signals were sensitive to the concentration of the odorant mixture, with a typical lifetime, shape, and adaptation profile as seen in responses upon repeated stimulation in vivo. We found that the rising time to the peak current was ∼161 ms, while the signal back to baseline was in 1.8 s, which are in agreement with the natural intracellular electrophysiological responses. These results provide the first evidence that mature OSNs grown in a planar triode device are able to detect direct electrophysiological responses to odorants.

AB - An artificial nose was developed to mimic aspects of sensory transduction of the peripheral mammalian olfactory system. We directly cultured and differentiated rat olfactory sensory neurons (OSNs) on indium-tin oxide electrodes of planar triode substrates without a coupling agent. Direct voltage (∼50 μV) and current (∼250 nA) signals were measured simultaneously when OSNs on the planar triode substrates were exposed to odorant mixtures. The response signals were sensitive to the concentration of the odorant mixture, with a typical lifetime, shape, and adaptation profile as seen in responses upon repeated stimulation in vivo. We found that the rising time to the peak current was ∼161 ms, while the signal back to baseline was in 1.8 s, which are in agreement with the natural intracellular electrophysiological responses. These results provide the first evidence that mature OSNs grown in a planar triode device are able to detect direct electrophysiological responses to odorants.

UR - http://www.scopus.com/inward/record.url?scp=84859340055&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84859340055&partnerID=8YFLogxK

U2 - 10.1039/c2an16205a

DO - 10.1039/c2an16205a

M3 - Article

C2 - 22434037

AN - SCOPUS:84859340055

VL - 137

SP - 2047

EP - 2053

JO - The Analyst

JF - The Analyst

SN - 0003-2654

IS - 9

ER -