Direct facile screening of recombinant DNA vector constructs

Research output: Contribution to journalArticle

Abstract

Direct efficient facile screening of bacterial transformants with the goal of selecting, retrieving, and using recombinant DNA is exemplified by simple visual-based colorimetric inspections or fluorescent protein-based assays. We describe pRedScript, which introduces the constitutive expression of a very bright red fluorescent protein into transformants. On agar plates, red colonies are simply visualized in ambient white light in stark contrast to recombinant transformants that are white. In addition, the bright red fluorescence of the reporter protein can also be harnessed as a sensitive signal for screening bacterial promoters during the development of optimized fermentation conditions.

Original languageEnglish (US)
Pages (from-to)1-3
Number of pages3
JournalAnalytical Biochemistry
Volume450
Issue number1
DOIs
StatePublished - Apr 1 2014

Fingerprint

Recombinant DNA
Screening
Fermentation
Agar
Assays
Proteins
Fluorescence
Inspection
Light
red fluorescent protein

Keywords

  • mCherry
  • Recombinant DNA
  • Red color
  • Screening

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

Direct facile screening of recombinant DNA vector constructs. / Winnard, Paul T; Challa, Rushi; Bhujwalla, Zaver M; Raman, Venu.

In: Analytical Biochemistry, Vol. 450, No. 1, 01.04.2014, p. 1-3.

Research output: Contribution to journalArticle

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