Direct evidence for homologous sequences on the paracentric regions of human chromosome 1

Bhushan D. Hardas, Ji Zhang, Jeffrey M. Trent, James T. Elder

Research output: Contribution to journalArticlepeer-review

Abstract

Calcyclin is a member of the S100 family of proteins, many of which are encoded by genes that have been localized to the proximal long arm of human chromosome 1 (bands q21-q22). A 450-kb yeast artificial chromosome clone containing the human calcyclin gene was identified by PCR screening and used as a probe for fluorescence in situ hybridization (FISH). Along with the expected hybridization to 1q21, simultaneous, specific hybridization to the centromeric region of the short arm of chromosome 1 was also observed. An identical pattern of hybridization was observed when microdissected 1q21 DNA sequences were used as a probe for FISH, confirming the presence of homologous sequences flanking both sides of the centromere of human chromosome 1. These results are consistent with a model in which human chromosome 1 arose by insertion of the centromere and heterochromatin into an ancestral chromosome containing chromosome-specific repetitive sequences.

Original languageEnglish (US)
Pages (from-to)359-363
Number of pages5
JournalGenomics
Volume21
Issue number2
DOIs
StatePublished - May 15 1994

ASJC Scopus subject areas

  • Genetics

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