Direct evidence for electron transfer from linoleic acid to triplet states of riboflavin and FAD: A laser photolysis study

Chang Yuan Lu, Guanshu Liu, Zhen Hui Han, Hong Fei Tang, Si De Yao, Nian Yun Lin, Jun Nan Chen, Yu Min Mao

Research output: Contribution to journalArticle

Abstract

Subcellular localization of ribofiavin (RF, Vitamin B2) in human normal liver L02 cells has been studied by means of fluorescence microscopy. The observed distribution of riboflavin inside the nucleus indicates for the first time that the photo - and radio - sensitized target is predominant inside the cells. While at higher concentrations, riboflavin can also be found in the cellular membrane, which demonstrates that cellular membrane is also a target site for riboflavin photo - and radio - sensitization. Here in this work, linoleic acid was used as a lipid model system in order to study the damaging potential of photoexcited flavin. By using time-resolved 308 run laser flash photolysis with transient absorbance detection, the formation process of radical anion of riboflavin (RF•-/RFH) or flavin adenine dinucleotide (FAD•-/FADH) was found to be synchronous with the decay of triplet states of riboflavin (3RF*) or flavin adenine dinucleotide (3FAD*). Moreover, the decay of 3RF* or 3FAD* was pseudo - first - order in the concentration of linoleic acid. These observations provide for the first time direct evidence of electron transfer from linoleic acid to the triplet states of riboflavin and flavin adenine dinucleotide.

Original languageEnglish (US)
Pages (from-to)1858-1863
Number of pages6
JournalActa Chimica Sinica
Volume59
Issue number11
StatePublished - 2001
Externally publishedYes

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Flavin-Adenine Dinucleotide
Riboflavin
Photolysis
Linoleic Acid
Electrons
Lasers
Membranes
Fluorescence microscopy
Liver
Anions
Lipids

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Lu, C. Y., Liu, G., Han, Z. H., Tang, H. F., Yao, S. D., Lin, N. Y., ... Mao, Y. M. (2001). Direct evidence for electron transfer from linoleic acid to triplet states of riboflavin and FAD: A laser photolysis study. Acta Chimica Sinica, 59(11), 1858-1863.

Direct evidence for electron transfer from linoleic acid to triplet states of riboflavin and FAD : A laser photolysis study. / Lu, Chang Yuan; Liu, Guanshu; Han, Zhen Hui; Tang, Hong Fei; Yao, Si De; Lin, Nian Yun; Chen, Jun Nan; Mao, Yu Min.

In: Acta Chimica Sinica, Vol. 59, No. 11, 2001, p. 1858-1863.

Research output: Contribution to journalArticle

Lu, CY, Liu, G, Han, ZH, Tang, HF, Yao, SD, Lin, NY, Chen, JN & Mao, YM 2001, 'Direct evidence for electron transfer from linoleic acid to triplet states of riboflavin and FAD: A laser photolysis study', Acta Chimica Sinica, vol. 59, no. 11, pp. 1858-1863.
Lu, Chang Yuan ; Liu, Guanshu ; Han, Zhen Hui ; Tang, Hong Fei ; Yao, Si De ; Lin, Nian Yun ; Chen, Jun Nan ; Mao, Yu Min. / Direct evidence for electron transfer from linoleic acid to triplet states of riboflavin and FAD : A laser photolysis study. In: Acta Chimica Sinica. 2001 ; Vol. 59, No. 11. pp. 1858-1863.
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