Direct demonstration of actin filament annealing in vitro

D. B. Murphy, R. O. Gray, W. A. Grasser, T. D. Pollard

Research output: Contribution to journalArticle

Abstract

Direct electron microscopic examination confirms that short actin filaments rapidly anneal end-to-end in vitro, leading over time to an increase in filament length at steady state. During annealing of mixtures of native unlabeled filaments and glutaraldehyde-fixed filaments labeled with myosin subfragment-1, the structural polarity within heteropolymers is conserved absolutely. Annealing does not appear to require either ATP hydrolysis or the presence of exogenous actin monomers, suggesting that joining occurs through the direct association of filament ends. During recovery from sonication the initial rate of annealing is consistent with a second-order reaction involving the collision of two filament ends with an apparent annealing rate constant of 107 M-1s-1. This rapid phase lasts <10 s and is followed by a slow phase lasting minutes to hours. Annealing is calculated to contribute minimally to filament elongation during the initial stages of self-assembly. However, the rapid rate of annealing of sonicated fixed filaments observed in vitro suggests that it may be an efficient mechanism for repairing breaks in filaments and that annealing together with polymer-severing mechanisms may contribute significantly to the dynamics and function of actin filaments in vivo.

Original languageEnglish (US)
Pages (from-to)1947-1954
Number of pages8
JournalJournal of Cell Biology
Volume106
Issue number6
DOIs
StatePublished - Jan 1 1988

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ASJC Scopus subject areas

  • Cell Biology

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