Direct demonstration of actin filament annealing in vitro

D. B. Murphy, Ronald H Gray, W. A. Grasser, T. D. Pollard

Research output: Contribution to journalArticle

Abstract

Direct electron microscopic examination confirms that short actin filaments rapidly anneal end-to-end in vitro, leading over time to an increase in filament length at steady state. During annealing of mixtures of native unlabeled filaments and glutaraldehyde-fixed filaments labeled with myosin subfragment-1, the structural polarity within heteropolymers is conserved absolutely. Annealing does not appear to require either ATP hydrolysis or the presence of exogenous actin monomers, suggesting that joining occurs through the direct association of filament ends. During recovery from sonication the initial rate of annealing is consistent with a second-order reaction involving the collision of two filament ends with an apparent annealing rate constant of 107 M-1s-1. This rapid phase lasts

Original languageEnglish (US)
Pages (from-to)1947-1954
Number of pages8
JournalJournal of Cell Biology
Volume106
Issue number6
StatePublished - 1988

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Myosin Subfragments
Sonication
Glutaral
Actin Cytoskeleton
Actins
Hydrolysis
Adenosine Triphosphate
Electrons
In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology

Cite this

Murphy, D. B., Gray, R. H., Grasser, W. A., & Pollard, T. D. (1988). Direct demonstration of actin filament annealing in vitro. Journal of Cell Biology, 106(6), 1947-1954.

Direct demonstration of actin filament annealing in vitro. / Murphy, D. B.; Gray, Ronald H; Grasser, W. A.; Pollard, T. D.

In: Journal of Cell Biology, Vol. 106, No. 6, 1988, p. 1947-1954.

Research output: Contribution to journalArticle

Murphy, DB, Gray, RH, Grasser, WA & Pollard, TD 1988, 'Direct demonstration of actin filament annealing in vitro', Journal of Cell Biology, vol. 106, no. 6, pp. 1947-1954.
Murphy DB, Gray RH, Grasser WA, Pollard TD. Direct demonstration of actin filament annealing in vitro. Journal of Cell Biology. 1988;106(6):1947-1954.
Murphy, D. B. ; Gray, Ronald H ; Grasser, W. A. ; Pollard, T. D. / Direct demonstration of actin filament annealing in vitro. In: Journal of Cell Biology. 1988 ; Vol. 106, No. 6. pp. 1947-1954.
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