Direct coupling of transcription and mRNA processing through the thermogenic coactivator PGC-1

María Monsalve, Zhidan Wu, Guillaume Adelmant, Pere Puigserver, Melina Fan, Bruce M. Spiegelman

Research output: Contribution to journalArticlepeer-review

Abstract

Transcription and mRNA processing are coupled events in vivo, but the mechanisms that coordinate these processes are largely unknown. PGC-1 is a transcriptional coactivator that plays a major role in the regulation of adaptive thermogenesis. PGC-1 also has certain motifs characteristic of splicing factors. We demonstrate here that mutations in the serine- and arginine-rich domain and RNA recognition motif of PGC-1 interfere with the ability of PGC-1 to induce mRNAs of target genes. These mutations also disrupt the ability of PGC-1 to co-localize and associate with RNA processing factors. PGC-1 can alter the processing of an mRNA, but only when it is loaded onto the promoter of the gene. These data demonstrate the coordinated regulation of RNA transcription and processing through PGC-1.

Original languageEnglish (US)
Pages (from-to)307-316
Number of pages10
JournalMolecular Cell
Volume6
Issue number2
StatePublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology

Fingerprint Dive into the research topics of 'Direct coupling of transcription and mRNA processing through the thermogenic coactivator PGC-1'. Together they form a unique fingerprint.

Cite this