Direct conversion of human myoblasts into brown-like adipocytes by engineered super-active PPARγ

Yanbei Zhu, Rongze Yang, John McLenithan, Daozhan Yu, Hong Wang, Yaping Wang, Devinder Singh, John Olson, Carole Sztalryd, Dalong Zhu, Da Wei Gong

Research output: Contribution to journalArticle

Abstract

Objective To determine whether super-activation of PPARγ can reprogram human myoblasts into brown-like adipocytes and to establish a new cell model for browning research. Methods To enhance the PPARγ signaling, M3, the transactivation domain of MyoD, was fused to PPARγ. PPARγ and M3-PPARγ-lentiviral vectors were used to convert human myoblasts into adipocytes. Brown adipocyte markers of the reprogrammed adipocytes were assessed by qPCR and protein analyses. White adipocytes differentiated from subcutaneous stromal vascular cells and perithyroid brown fat tissues were used as references. Results In transient transfections, M3-PPARγ had a stronger constitutive activity than PPARγ by reporter assay. Although the transduction of either PPARγ or M3-PPARγ induced adipogenesis in myoblasts, M3-PPARγ drastically induced the brown adipocyte markers of UCP1, CIDEA, and PRDM16 by 1,050, 2.4, and 5.0 fold, respectively and increased mitochondria contents by 4 fold, compared to PPARγ. Conclusions Super-activation of PPARγ can effectively convert human myoblasts into brown-like adipocytes and a new approach to derive brown-like adipocytes.

Original languageEnglish (US)
Pages (from-to)1014-1021
Number of pages8
JournalObesity
Volume23
Issue number5
DOIs
StatePublished - May 1 2015
Externally publishedYes

Fingerprint

Brown Adipocytes
Peroxisome Proliferator-Activated Receptors
Myoblasts
Adipocytes
White Adipocytes
Adipogenesis
Brown Adipose Tissue
Stromal Cells
Transcriptional Activation
Transfection
Blood Vessels

ASJC Scopus subject areas

  • Endocrinology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Zhu, Y., Yang, R., McLenithan, J., Yu, D., Wang, H., Wang, Y., ... Gong, D. W. (2015). Direct conversion of human myoblasts into brown-like adipocytes by engineered super-active PPARγ. Obesity, 23(5), 1014-1021. https://doi.org/10.1002/oby.21062

Direct conversion of human myoblasts into brown-like adipocytes by engineered super-active PPARγ. / Zhu, Yanbei; Yang, Rongze; McLenithan, John; Yu, Daozhan; Wang, Hong; Wang, Yaping; Singh, Devinder; Olson, John; Sztalryd, Carole; Zhu, Dalong; Gong, Da Wei.

In: Obesity, Vol. 23, No. 5, 01.05.2015, p. 1014-1021.

Research output: Contribution to journalArticle

Zhu, Y, Yang, R, McLenithan, J, Yu, D, Wang, H, Wang, Y, Singh, D, Olson, J, Sztalryd, C, Zhu, D & Gong, DW 2015, 'Direct conversion of human myoblasts into brown-like adipocytes by engineered super-active PPARγ', Obesity, vol. 23, no. 5, pp. 1014-1021. https://doi.org/10.1002/oby.21062
Zhu, Yanbei ; Yang, Rongze ; McLenithan, John ; Yu, Daozhan ; Wang, Hong ; Wang, Yaping ; Singh, Devinder ; Olson, John ; Sztalryd, Carole ; Zhu, Dalong ; Gong, Da Wei. / Direct conversion of human myoblasts into brown-like adipocytes by engineered super-active PPARγ. In: Obesity. 2015 ; Vol. 23, No. 5. pp. 1014-1021.
@article{ba161458b79e4a049a8f81adca1faf6c,
title = "Direct conversion of human myoblasts into brown-like adipocytes by engineered super-active PPARγ",
abstract = "Objective To determine whether super-activation of PPARγ can reprogram human myoblasts into brown-like adipocytes and to establish a new cell model for browning research. Methods To enhance the PPARγ signaling, M3, the transactivation domain of MyoD, was fused to PPARγ. PPARγ and M3-PPARγ-lentiviral vectors were used to convert human myoblasts into adipocytes. Brown adipocyte markers of the reprogrammed adipocytes were assessed by qPCR and protein analyses. White adipocytes differentiated from subcutaneous stromal vascular cells and perithyroid brown fat tissues were used as references. Results In transient transfections, M3-PPARγ had a stronger constitutive activity than PPARγ by reporter assay. Although the transduction of either PPARγ or M3-PPARγ induced adipogenesis in myoblasts, M3-PPARγ drastically induced the brown adipocyte markers of UCP1, CIDEA, and PRDM16 by 1,050, 2.4, and 5.0 fold, respectively and increased mitochondria contents by 4 fold, compared to PPARγ. Conclusions Super-activation of PPARγ can effectively convert human myoblasts into brown-like adipocytes and a new approach to derive brown-like adipocytes.",
author = "Yanbei Zhu and Rongze Yang and John McLenithan and Daozhan Yu and Hong Wang and Yaping Wang and Devinder Singh and John Olson and Carole Sztalryd and Dalong Zhu and Gong, {Da Wei}",
year = "2015",
month = "5",
day = "1",
doi = "10.1002/oby.21062",
language = "English (US)",
volume = "23",
pages = "1014--1021",
journal = "Obesity",
issn = "1930-7381",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Direct conversion of human myoblasts into brown-like adipocytes by engineered super-active PPARγ

AU - Zhu, Yanbei

AU - Yang, Rongze

AU - McLenithan, John

AU - Yu, Daozhan

AU - Wang, Hong

AU - Wang, Yaping

AU - Singh, Devinder

AU - Olson, John

AU - Sztalryd, Carole

AU - Zhu, Dalong

AU - Gong, Da Wei

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Objective To determine whether super-activation of PPARγ can reprogram human myoblasts into brown-like adipocytes and to establish a new cell model for browning research. Methods To enhance the PPARγ signaling, M3, the transactivation domain of MyoD, was fused to PPARγ. PPARγ and M3-PPARγ-lentiviral vectors were used to convert human myoblasts into adipocytes. Brown adipocyte markers of the reprogrammed adipocytes were assessed by qPCR and protein analyses. White adipocytes differentiated from subcutaneous stromal vascular cells and perithyroid brown fat tissues were used as references. Results In transient transfections, M3-PPARγ had a stronger constitutive activity than PPARγ by reporter assay. Although the transduction of either PPARγ or M3-PPARγ induced adipogenesis in myoblasts, M3-PPARγ drastically induced the brown adipocyte markers of UCP1, CIDEA, and PRDM16 by 1,050, 2.4, and 5.0 fold, respectively and increased mitochondria contents by 4 fold, compared to PPARγ. Conclusions Super-activation of PPARγ can effectively convert human myoblasts into brown-like adipocytes and a new approach to derive brown-like adipocytes.

AB - Objective To determine whether super-activation of PPARγ can reprogram human myoblasts into brown-like adipocytes and to establish a new cell model for browning research. Methods To enhance the PPARγ signaling, M3, the transactivation domain of MyoD, was fused to PPARγ. PPARγ and M3-PPARγ-lentiviral vectors were used to convert human myoblasts into adipocytes. Brown adipocyte markers of the reprogrammed adipocytes were assessed by qPCR and protein analyses. White adipocytes differentiated from subcutaneous stromal vascular cells and perithyroid brown fat tissues were used as references. Results In transient transfections, M3-PPARγ had a stronger constitutive activity than PPARγ by reporter assay. Although the transduction of either PPARγ or M3-PPARγ induced adipogenesis in myoblasts, M3-PPARγ drastically induced the brown adipocyte markers of UCP1, CIDEA, and PRDM16 by 1,050, 2.4, and 5.0 fold, respectively and increased mitochondria contents by 4 fold, compared to PPARγ. Conclusions Super-activation of PPARγ can effectively convert human myoblasts into brown-like adipocytes and a new approach to derive brown-like adipocytes.

UR - http://www.scopus.com/inward/record.url?scp=84928564547&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84928564547&partnerID=8YFLogxK

U2 - 10.1002/oby.21062

DO - 10.1002/oby.21062

M3 - Article

C2 - 25919922

AN - SCOPUS:84928564547

VL - 23

SP - 1014

EP - 1021

JO - Obesity

JF - Obesity

SN - 1930-7381

IS - 5

ER -