Direct analysis of viral-specific CD8+ T cells with soluble HLA- A2/Tax11-19 tetramer complexes in patients with human T cell lymphotropic virus-associated myelopathy

Katarzyna Bieganowska, Per Höllsberg, Guy J. Buckle, Dong Gyun Lim, Tim F. Greten, Jonathan Schneck, John D. Altman, Steven Jacobson, Stephen L. Ledis, Barrie Hanchard, Jonathan Chin, Owen Morgan, Patricia A. Roth, David A. Hafler

Research output: Contribution to journalArticlepeer-review

Abstract

Human T cell lymphotropic virus-I (HTLV-I)-associated myelopathy is a slowly progressive neurologic disease characterized by inflammatory infiltrates in the central nervous system accompanied by clonal expansion of HTLV-I-reactive CD8+ T-cells. In patients carrying the HLA-A2 allele, the immune response is primarily directed to the Tax1 1-19 peptide. The frequency, activation state, and TCR usage of HLA-A2/Tax11-19 binding T cells in patients with HTLV-I-associated myelopathy was determined using MHC class I tetramers loaded with the Tax11-19 peptide. Circulating Tax11-19-reactive T cells were found at very high frequencies, approaching 1:10 circulating CD8+ T cells. T cells binding HLA-A2/Tax11-19 consisted of heterogeneous populations expressing different chemokine receptors and the IL-2R β-chain but not the IL-2R α-chain. Additionally, Tax11-19-reactive CD8+ T cells used one predominant TCR Vβ-chain for the recognition of the HLA-A2/Tax11- 19 complex. These data provide direct evidence for high frequencies of circulating Tax11-19-reactive CD8+ T cells in patients with HTLV-1- associated myelopathy.

Original languageEnglish (US)
Pages (from-to)1765-1771
Number of pages7
JournalJournal of Immunology
Volume162
Issue number3
StatePublished - Feb 1 1999

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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