Diphenhydramine determination in human plasma by gas–liquid chromatography using nitrogen–phosphorus detection: Application to single low‐dose pharmacokinetic studies

Darrell R. Abernethy; David J. Greenblatt

doi:10.1002/jps.2600720825

Diphenhydramine determination in human plasma by gas–liquid chromatography using nitrogen–phosphorus detection: Application to single low‐dose pharmacokinetic studies

Darrell R. Abernethy, David J. Greenblatt

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

A highly sensitive method (∼1 ng/ml) for single‐dose pharmacokinetic studies of diphenhydramine which utilizes GLC with nitrogen–phosphorus detection is described. Standard curves, using orphenadrine as the internal standard, were linear for diphenhydramine concentrations from 1.0 to 300 ng/ml. Applicability of the method was demonstrated by a pharmacokinetic study in a normal volunteer who received 25 mg iv of diphenhydramine.

Original language	English (US)
Pages (from-to)	941-943
Number of pages	3
Journal	Journal of Pharmaceutical Sciences
Volume	72
Issue number	8
DOIs	https://doi.org/10.1002/jps.2600720825
State	Published - Aug 1983

Keywords

Diphenhydramine—determination in human plasma using GLC with nitrogen–phosphorus detection, application to single low‐dose pharmacokinetic studies
GLC—with nitrogen–phosphorus detection, determination of diphenhydramine in human plasma, single‐dose pharmacokinetic study
Pharmacokinetics—of diphenhydramine in the human, detection after a single dose using GLC with nitrogen–phosphorus detection

ASJC Scopus subject areas

Pharmaceutical Science

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10.1002/jps.2600720825

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title = "Diphenhydramine determination in human plasma by gas–liquid chromatography using nitrogen–phosphorus detection: Application to single low‐dose pharmacokinetic studies",

abstract = "A highly sensitive method (∼1 ng/ml) for single‐dose pharmacokinetic studies of diphenhydramine which utilizes GLC with nitrogen–phosphorus detection is described. Standard curves, using orphenadrine as the internal standard, were linear for diphenhydramine concentrations from 1.0 to 300 ng/ml. Applicability of the method was demonstrated by a pharmacokinetic study in a normal volunteer who received 25 mg iv of diphenhydramine.",

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author = "Abernethy, {Darrell R.} and Greenblatt, {David J.}",

year = "1983",

month = aug,

doi = "10.1002/jps.2600720825",

language = "English (US)",

volume = "72",

pages = "941--943",

journal = "Journal of Pharmaceutical Sciences",

issn = "0022-3549",

publisher = "John Wiley and Sons Inc.",

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T1 - Diphenhydramine determination in human plasma by gas–liquid chromatography using nitrogen–phosphorus detection

T2 - Application to single low‐dose pharmacokinetic studies

AU - Abernethy, Darrell R.

AU - Greenblatt, David J.

PY - 1983/8

Y1 - 1983/8

N2 - A highly sensitive method (∼1 ng/ml) for single‐dose pharmacokinetic studies of diphenhydramine which utilizes GLC with nitrogen–phosphorus detection is described. Standard curves, using orphenadrine as the internal standard, were linear for diphenhydramine concentrations from 1.0 to 300 ng/ml. Applicability of the method was demonstrated by a pharmacokinetic study in a normal volunteer who received 25 mg iv of diphenhydramine.

AB - A highly sensitive method (∼1 ng/ml) for single‐dose pharmacokinetic studies of diphenhydramine which utilizes GLC with nitrogen–phosphorus detection is described. Standard curves, using orphenadrine as the internal standard, were linear for diphenhydramine concentrations from 1.0 to 300 ng/ml. Applicability of the method was demonstrated by a pharmacokinetic study in a normal volunteer who received 25 mg iv of diphenhydramine.

KW - Diphenhydramine—determination in human plasma using GLC with nitrogen–phosphorus detection, application to single low‐dose pharmacokinetic studies

KW - GLC—with nitrogen–phosphorus detection, determination of diphenhydramine in human plasma, single‐dose pharmacokinetic study

KW - Pharmacokinetics—of diphenhydramine in the human, detection after a single dose using GLC with nitrogen–phosphorus detection

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Diphenhydramine determination in human plasma by gas–liquid chromatography using nitrogen–phosphorus detection: Application to single low‐dose pharmacokinetic studies

Abstract

Keywords

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