Diphenhydramine determination in human plasma by gas–liquid chromatography using nitrogen–phosphorus detection: Application to single low‐dose pharmacokinetic studies

Darrell R. Abernethy, David J. Greenblatt

Research output: Contribution to journalArticle


A highly sensitive method (∼1 ng/ml) for single‐dose pharmacokinetic studies of diphenhydramine which utilizes GLC with nitrogen–phosphorus detection is described. Standard curves, using orphenadrine as the internal standard, were linear for diphenhydramine concentrations from 1.0 to 300 ng/ml. Applicability of the method was demonstrated by a pharmacokinetic study in a normal volunteer who received 25 mg iv of diphenhydramine.

Original languageEnglish (US)
Pages (from-to)941-943
Number of pages3
JournalJournal of Pharmaceutical Sciences
Issue number8
StatePublished - Aug 1983



  • Diphenhydramine—determination in human plasma using GLC with nitrogen–phosphorus detection, application to single low‐dose pharmacokinetic studies
  • GLC—with nitrogen–phosphorus detection, determination of diphenhydramine in human plasma, single‐dose pharmacokinetic study
  • Pharmacokinetics—of diphenhydramine in the human, detection after a single dose using GLC with nitrogen–phosphorus detection

ASJC Scopus subject areas

  • Pharmaceutical Science

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