Dimples, pores, star-rings, and thin rings on growing nuclear envelopes: Evidence for structural intermediates in nuclear pore complex assembly

Martin W. Goldberg, Christiane Wiese, Terence D. Allen, Katherine Lee Wilson

Research output: Contribution to journalArticle

Abstract

We used field emission in-lens scanning electron microscopy to examine newly-assembled, growing nuclear envelopes in Xenopus egg extracts. Scattered among nuclear pore complexes were rare 'dimples' (outer membrane depressions, 5-35 nm diameter), more abundant holes (pores) with a variety of edge geometries (35-45 nm diameter; 3.3% of structures), pores containing one to eight triangular 'star-ring' subunits (2.1% of total), and more complicated structures. Neither mature complexes, nor these novel structures, formed when wheat germ agglutinin (which binds O-glycosylated nucleoporins) was added at high concentrations (> 500 μg/ml) directly to the assembly reaction; low concentrations (10 μg/ml) had no effect. However at intermediate concentrations (50-100 μg/ml), wheat germ agglutinin caused a dramatic, sugar-reversible accumulation of 'empty' pores, and other structures; this effect correlated with the lectin-induced precipitation of a variable proportion of each major Xenopus wheat-germ-agglutinin-binding nucleoporin. Another inhibitor, dibromo-BAPTA (5',5'-dibromo-1,2-bis[o-aminophenoxy]ethane-N,N,N',N'-tetraacetic acid), had different effects depending on its time of addition to the assembly reaction. When 1 mM dibromo-BAPTA was added at time zero, no pore-related structures formed. However, when dibromo-BAPTA was added to growing nuclei 40-45 minutes after initiating assembly, star-rings and other structures accumulated, suggesting that dibromo-BAPTA can inhibit multiple stages in pore complex assembly. We propose that assembly begins with the formation and stabilization of a hole (pore) through the nuclear envelope, and that dimples, pores, star-rings, and thin rings are structural intermediates in nuclear pore complex assembly.

Original languageEnglish (US)
Pages (from-to)409-420
Number of pages12
JournalJournal of Cell Science
Volume110
Issue number4
StatePublished - Feb 1997

Fingerprint

Nuclear Pore
Nuclear Envelope
Wheat Germ Agglutinins
Nuclear Pore Complex Proteins
Xenopus
Lectins
Electron Scanning Microscopy
Lenses
Ovum
Membranes
5,5'-dibromo-1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid

Keywords

  • FEISEM
  • Nuclear envelope
  • Nuclear pore complex

ASJC Scopus subject areas

  • Cell Biology

Cite this

Dimples, pores, star-rings, and thin rings on growing nuclear envelopes : Evidence for structural intermediates in nuclear pore complex assembly. / Goldberg, Martin W.; Wiese, Christiane; Allen, Terence D.; Wilson, Katherine Lee.

In: Journal of Cell Science, Vol. 110, No. 4, 02.1997, p. 409-420.

Research output: Contribution to journalArticle

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