The effect of advanced age on the response of active tension, maximal rate of tension development (dT/dt) and contraction duration to catecholamines and to calcium was evaluated in isometric trabeculae carneae from young adult (6 mth), middle aged (12 mth), and aged (25 mth) rats. Control values were not age dependent except for that for contraction duration which was prolonged in the aged group. At a norepinephrine concentration of 8 x 10-5M, dT/dt increased to 163.8±5.3% of control in the young adult group and to 125.9±6.3% of control in the aged group (P<0.001). Active tension increased to 121.3±4.0% of control in the young adult muscles but did not increase in the aged muscles (P<0.01). Contraction duration shortened proportionately in both age groups. Similar results were obtained with isoproterenol. In contrast to the response to catecholamines, there was no age difference in the response of active tension and dT/dt to increasing concentrations of calcium. It is concluded that the intrinsic inotropic response to catecholamines is diminished in the aged myocardium. This finding does not appear to result from differences in tachyphylaxis, tissue uptake of catecholamines or the ability of the contractile proteins to respond to increasing concentrations of calcium, but instead may result from a decreased ability of catecholamines to increase the intracellular calcium available for contraction.
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine