Dimethyl sulfoxide suppresses NMDA- and AMPA-induced ion currents and calcium influx and protects against excitotoxic death in hippocampal neurons

Chengbiao Lu, Mark P. Mattson

Research output: Contribution to journalArticlepeer-review

Abstract

Dimethyl sulfoxide (DMSO) is widely used in neuroscience research as a solvent for various pharmacological agents in both cell culture and in vivo studies and is also used in humans to treat musculoskeletal problems and pain. We now report that concentrations of DMSO to which neurons are typically exposed in experimental studies and in human patients (0.5-1.5%) inhibit glutamate responses in hippocampal neurons. DMSO suppresses, in a rapidly reversible manner, electrophysiological responses and calcium influx induced by glutamate, N-methyl-D-aspartate, and α-amino-3-hydroxy-5-methylisoxazole-4-propionate. Moreover, DMSO can prevent excitotoxic death of the neurons. These findings have important implications for the use of DMSO as a solvent in studies that involve glutamatergic neurotransmission. Our data also identify a mechanism that might explain clinical effects of DMSO on both peripheral and CNS neurons and suggest a potential use for DMSO in the treatment of excitotoxic neurodegenerative conditions.

Original languageEnglish (US)
Pages (from-to)180-185
Number of pages6
JournalExperimental Neurology
Volume170
Issue number1
DOIs
StatePublished - 2001

Keywords

  • DMSO
  • Fura-2
  • Glutamate
  • Ischemia
  • Pain
  • Patch clamp

ASJC Scopus subject areas

  • Neurology
  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Dimethyl sulfoxide suppresses NMDA- and AMPA-induced ion currents and calcium influx and protects against excitotoxic death in hippocampal neurons'. Together they form a unique fingerprint.

Cite this